BenchStable™ RPMI 1640
BenchStable™ RPMI 1640
Gibco™

BenchStable™ RPMI 1640

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Gibco RPMI 1640 培养基 (Roswell Park Memorial Institute 1640 Medium)了解更多信息
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货号数量
A419230210 x 500 mL
A4192301500 mL
货号 A4192302
价格(CNY)
3,589.19
飞享价
Ends: 31-Dec-2025
4,545.00
共减 955.81 (21%)
Each
添加至购物车
数量:
10 x 500 mL
价格(CNY)
3,589.19
飞享价
Ends: 31-Dec-2025
4,545.00
共减 955.81 (21%)
Each
添加至购物车
Gibco RPMI 1640 培养基 (Roswell Park Memorial Institute 1640 Medium) 起初是为了悬浮培养人白血病单层细胞而开发的。此后,RPMI 1640 培养基被发现适用于多种哺乳动物细胞,包括 HeLa 细胞、Jurkat 细胞、MCF-7 细胞、PC12 细胞、PBMC 细胞、星形胶质细胞和癌细胞。针对广泛的细胞培养应用,提供了多种组分的 RPMI 1640 改良培养基。使用培养基选择工具查找适合的配方。

BenchStable RPMI 1640 培养基经过专门设计,可以在室温下储存,对细胞增殖和最大细胞密度的支持效果可媲美添加 ≥10% 胎牛血清 (FBS) 的常规 RPMI 1640 培养基。

这种 RPMI 1640 培养基的改良方式如下:
•针对室温稳定性进行了优化
•包含 GlutaMAX、酚红
•不含 HEPES

BenchStable RPMI 1640 培养基的使用
RPMI 1640 培养基相比其他培养基之所以具有独特的效果,是因为其中含有还原剂谷胱甘肽和高浓度的维生素。RPMI 1640 培养基含有生物素、维生素 B12 以及 PABA,这些成分是 Eagle's MEM 和 DMEM 所不具备的。此外,该培养基之中还含有高浓度的维生素肌醇和胆碱。RPMI 1640 培养基使用碳酸氢钠缓冲体系 (2.0 g/L),因此需要 5–10% CO2 的环境来维持生理 pH 值。含有 GlutaMAX 添加剂的 RPMI 1640 培养基具有易于使用的包装形式,可显著减少有毒氨的积累,并改善细胞活力和生长情况。BenchStable RPMI 1640 培养基不含蛋白质、脂质或生长因子,因此需要补充 ≥10% 胎牛血清 (FBS)

cGMP 生产和质量体系
Gibco BenchStable 培养基在位于纽约格兰德岛的符合 cGMP 要求的工厂内生产。该工厂是在 FDA 登记的医疗器械生产商,且通过 ISO 13485 标准认证。

有关 Gibco BenchStable 培养基的更多信息,请访问 thermofisher.cn/benchstable

仅供科研使用。不可用于诊断程序。
规格
细胞系HeLa、Jurkat、MCF-7、PC-12、PBMC、星形胶质细胞和癌细胞
最大浓度1 X
环保功能节能、使用的资源极少、可持续包装
生产质量cGMP-compliant under the ISO 9001: 2015 standard
产品线BenchStable™
产品类型RPMI 1640 培养基(Roswell Park Memorial Institute 1640 培养基)
数量10 x 500 mL
有效期自生产之日起 12 个月
运输条件室温
分类非动物源性
形式液体
血清水平标准血清
无菌无菌过滤
加有添加剂低糖, GlutaMAX
不加添加剂不含 HEPES, 不含丙酮酸钠
Unit SizeEach
内容与储存
在室温下储存。避光。

常见问题解答 (FAQ)

Is it okay if I supplement BenchStable media with more than 10% FBS?

We recommended supplementing BenchStable media with at least 10% FBS for best results. If your culture method calls for greater than 10% FBS supplementation using our other existing media, we recommend that you use the same concentration with BenchStable media.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Is it okay if I supplement BenchStable media with 5% FBS?

We recommend supplementing BenchStable media with at least 10% FBS for best results. We have run tests on limited cell types using 5% FBS concentration and have not observed compromised performance. Please contact Tech Support at techsupport@thermofisher.com to ensure that your specific application allows for 5% serum supplementation.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Which cell lines have been tested in BenchStable media?

We have tested our BenchStable media for growth and morphology on the following cell lines: A549, CHO-K1, HEK293, HeLa, HL-60, Jurkat, MCF7, MDA-MB-231, Neuro2A, SH-SY5Y, and THP-1.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Do you offer BenchStable media without phenol red?

At this time, we do not offer BenchStable media without phenol red.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Will the shelf-life of BenchStable media improve if stored at lower temperature (2-8 degrees C)?

The shelf life of BenchStable media is unchanged when stored cold (2-4 degrees C) or ambient (15-30 degrees C).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.