CTS™ StemPro™ HSC Expansion Medium - FAQs

查看更多产品信息 CTS™ StemPro™ HSC Expansion Medium - FAQs (A4222301)

4 个常见问题解答

Can I use CTS StemPro HSC Expansion Medium without cytokines or growth factors?

This media system is formulated without cytokines or growth factors, but cytokines and/or growth factors should be added immediately prior to use. For hematopoietic stem and progenitor cell culture, we recommend adding the following cytokines at the final concentration indicated: SCF (100 ng/mL), FLT-3L (100 ng/mL), TPO (100 ng/mL), IL-3 (50 ng/mL), and IL-6 (20 ng/mL).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Which cell types have you tested with CTS StemPro HSC Expansion Medium?

CTS StemPro HSC Expansion Medium has been shown to expand healthy human CD34+ cells from cord blood and bone marrow, and mobilized peripheral blood from single and pooled donors. We have also demonstrated successful reprogramming of PBMC into iPSC using CTS StemPro HSC Expansion Medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can I use CTS StemPro HSC Expansion Medium to culture mouse HSCs?

We have not evaluated CTS StemPro HSC Expansion Medium for culture of mouse HSCs.

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Can I freeze 1X complete CTS StemPro HSC Expansion Medium to extend the product shelf life?

No, complete StemPro HSC Expansion Medium should not be frozen. The best results are observed when the complete medium is prepared on the day of use.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.