MagMAX™ Microbiome Ultra Nucleic Acid Isolation Kit, with bead tubes - FAQs

View additional product information for MagMAX™ Microbiome Ultra Nucleic Acid Isolation Kit, with bead tubes - FAQs (A42358)

8 product FAQs found

What are the bead beating options available for the MagMAX Microbiome Ultra Nucleic Acid Isolation Kit?

Here are the different bead-beating options that can be used:

- Omni Bead Ruptor 96: 30 Hz for 2 min
- Mini Bead Beater 96: 2 min
- Bead Bug: 30 sec is enough but for higher yields, use for 4 min at 4 m/s
- Plate Shaker (Vortex with plate adapter): 5 min at 2,000 rpm
- Vortex with 24 tube adapter: 10 min at 2,500 rom

What types of downstream analysis are compatible with the MagMAX Microbiome Ultra Nucleic Acid Isolation Kit?

The following downstream analysis are compatible with the MagMAX Microbiome Ultra Nucleic Acid Isolation Kit: PCR, qPCR, qRT-PCR, OpenArray analysis, microarray analysis, and next-generation sequencing.

What are the throughput options available for the MagMAX Microbiome Ultra Nucleic Acid Isolation Kit?

The MagMAX Microbiome Ultra Nucleic Acid Isolation Kit is available in two throughput formats: with bead tubes for low throughput and with bead plates for high throughput (96 samples at a time).

How long is the workflow for the MagMAX Microbiome Ultra Nucleic Acid Isolation Kit?

For fecal samples, the complete workflow takes 50 min and for all other sample types, it takes 70 min.

What are my options to obtain DNA only or RNA only using the MagMAX Microbiome Ultra Nucleic Acid Isolation Kit?

For DNA only: Add 10 µL of RNase A (Cat. No. AM2270, AM2271) in each well of the Wash 1 and Wash 2 plates.

For RNA only: DNA-free DNA Removal Kit (Cat. No. AM1906) can be used with purified total nucleic acid.

Is the purified DNA/RNA from the MagMAX Microbiome Ultra Nucleic Acid Isolation Kit completely inhibitor-free?

The MagMAX Microbiome Ultra Nucleic Acid Isolation Kit is based on a newly developed buffer system, allowing elimination of the vast majority of diverse inhibitors of enzymatic reactions, even from the most challenging samples such as stool and soil. In very rare instances, if some inhibitors still remain within the purified DNA/RNA, the sample can be diluted 10-20X prior to downstream PCR or other reactions.

Does the MagMAX Microbiome Ultra Nucleic Acid Isolation Kit efficiently lyse gram-positive bacteria and challenging fungal species?

The kit utilizes a combination of chemical and mechanical (bead beating) lysis strategies, which enables efficient lysis and DNA/RNA recovery from even the most challenging microbial species.

Which sample types can be processed with the MagMAX Microbiome Ultra Nucleic Acid Isolation Kit (Cat. Nos. A42357, A42358)?

This kit was developed for fast and efficient isolation of inhibitor-free microbial DNA/RNA from human stool, swabs (fecal, skin, buccal), samples in viral transport media (VTM), and body fluids (urine, saliva). It has also been confirmed to perform well with stool samples from other mammals, reptiles, fish, bird, and other samples like soil, bacterial cultures, and yeast cultures.

Considering that stool and soil are the most challenging sample types in terms of homogenization and depletion of inhibitors, the kit should work well for most other sample types that researchers might be processing in the lab.