TrueTag™ Donor DNA Kit, GFP - FAQs

My TrueTag Donor DNA Kit, GFP (Cat No. A42992) does not have the ACTB sgRNA included in the kit. Where can I get the ACTB sgRNA?

While the TrueTag Donor DNA Kit, GFP (Cat No: A42992) contains all the necessary reagents to generate the positive control donor template to tag ACTB at the N-terminal end with GFP, the gRNA targeting ACTB is not included in the kit. You would need to purchase it separately as a custom TrueGuide sgRNA that can be ordered through the Invitrogen TrueGuide gRNA ordering tool . The targeting sequence of the ACTB gRNA would be: CGCGGCGATATCATCATCCA.