Targeted In Situ Protein Diversification and Intra-organelle Validation in Mammalian Cells.
AuthorsErdogan M, Fabritius A, Basquin J, Griesbeck O
JournalCell Chem Biol
PubMed ID32142629
Engineered proteins must be phenotypically selected for function in the appropriate physiological context. Here, we present a versatile approach that allows generating panels of mammalian cells that express diversified heterologous protein libraries in the cytosol or subcellular compartments under stable conditions and in a single-variant-per-cell manner. To this end we adapt ... More
Effects of Prenatal Phthalate Exposure and Childhood Exercise on Maternal Behaviors in Female Rats at Postpartum: A Role of
AuthorsLee YJ, Lin HT, Chaudhary MA, Lee YC, Wang DC
JournalInt J Mol Sci
PubMed ID34576011
Both the detrimental effect of prenatal exposure to di-(2-ethylhexyl)-phthalate (DEHP) and the beneficial effects of physical exercise on brain functions have been reported. The oxytocin pathway has been implicated in the onset of maternal behaviors. Epigenetic modification of the oxytocin receptor gene (OXTR) through DNA methylation has been associated with ... More