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View additional product information for iBright™ CL1500 Imaging System - FAQs (A44114, A44240CFR, A44240)
24 product FAQs found
iBright Imaging Systems support total lane protein-based normalization and housekeeping gene (HKP)-based normalization. To support the methods best suited for your experiment, iBright Imaging Systems and iBright Analysis Software provide multiple quantitation and normalization options to monitor or mathematically compensate for experimental or sample variability.
To learn more about total lane protein normalization, explore our application note.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
The iBright Imaging Systems support Ethernet network connections. WIFI is also supported with the addition of a USB-WIFI dongle. Data may be exported from the instrument via network and WIFI connections. It can also be exported via USB port.
For additional information on iBright functionality, visit this page.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
The iBright FL1500 Imaging System supports a wide range of dyes, stains and fluorophores in the visible and NIR wavelength range.
For more information on compatible reagents and applications, visit this page.
To learn more about the iBright system-supported fluorophores and installed filters and light sources, visit Fluorescence SpectraViewer.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
iBright Imaging Systems feature a built-in computer interface controlled by the 12.1 inch touchscreen. No additional computer is needed to operate the instrument.
For additional information on iBright functionality, visit this page.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
The iBright Imaging Systems utilize a transilluminator based on green LEDs, which effectively excite popular DNA dyes such ethidium bromide and SYBR Green dye and offer several additional benefits:
- No harmful UV rays: While UV light effectively excites many fluorescent dyes and stains, UV light is a health hazard. Further, prolonged exposure to UV light can damage DNA samples, and compromise the integrity of samples to be used for downstream applications, such as subcloning.
- No mercury waste: UV transilluminator bulbs may contain mercury, a hazardous substance, and therefore require special care for handling and disposal.
- Longer lifetime: LED bulbs have a substantially longer real-time life than fluorescent UV bulbs, which can add up to considerable cost savings over the lifetime of the instrument.
For additional information on iBright functionality, visit this page.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
The iBright FL1500 and CL1500 Imaging Systems include a suite of powerful automation tools to facilitate easy image capture and improve laboratory efficiency. These features include:
- Smart Exposure: this technology rapidly determines the optimal exposure time, minimizing the potential for over- or under-exposed images and the need to repeat exposures to get the desired signal. Smart exposure is enabled by built-in algorithms which aim to maximize sensitivity without creating band saturation.
- Automatic Mechanical Sample Rotation: The imager senses the edge of the sample and will physically rotate the turntable up to 10 degrees to straighten the sample prior to imaging.
- Automatic Zoom and Focus: After the sample is inserted in the imager, the system automatically optimizes the zoom and focus levels to allow for fast easy image capture and optimized image resolution.
For additional information on iBright functionality, visit this page.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
We offer three different iBright Imaging System models, the iBright FL1500, CL1500, and CL750 iBright Imaging Systems.
To learn more about the capabilities of these three iBright imager models, visit this page.
To speak with a representative about the iBright imager or request a quote or demo, please fill out this form.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
iBright imagers support the export of .tiff files in 8 and 16 bit formats. They also allow users to export images as .jpg and the proprietary .g2i file types. The iBright Analysis software primarily uses .g2i files as this format contains the most data to facilitate easy analysis. But it also supports .tiff files import and analysis.
For additional information on iBright functionality, visit this page.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
The iBright Imaging Systems support a wide range of dyes, stains, and fluorophores from the visible to NIR wavelength range.
For more information on compatible reagents and applications, visit this page.
To learn more about the iBright system-supported fluorophores and installed filters and light sources, visit Fluorescence SpectraViewer.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
The imaging area of the iBright Imaging system is 22.5 x 18.0 cm. This gives ample space for imaging 4 mini blots (or 2 midi blots) simultaneously.
For more information on iBright features and capabilities, visit this page.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
iBright imagers include a 12.1 inch LCD touchscreen interface.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
The free iBright Analysis Software (iBA) supports analysis of all images captured on iBright imagers and is available for free download on our website. There is also a Cloud version of iBA software available for users who prefer to work directly from a web browser.
The iBA software is supported on both Windows and Mac computers and can be used by any lab personnel who need it. This software automates data analysis and makes annotation, analysis, and reporting simple. To learn more about the iBA software, view the iBright Analysis Software how-to-videos available on this page.
To speak with a representative about the iBright imager or request a quote or demo, please fill out this form.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
The iBright CL1500 Imaging System supports a wide range of applications including the following:
Protein gel: colorimetric staining of gels (e.g., Coomassie, silver) and membranes (e.g., Ponceau S, Thermo Scientific Pierce Reversible Protein Stain), fluorescent staining of gels (e.g., Invitrogen SYPRO Ruby stain)
Nucleic acid gel: ethidium bromide and Invitrogen SYBR stains; imaging of E-Gels and hand pour gels is supported
Chemiluminescent western blot: chemiluminescence using all popular HRP and AP substrates (e.g., Thermo Scientific SuperSignal and Invitrogen WesternBreeze substrates)
Additional applications: colony counting for GFP, crystal violet, and blue/white colonies; TLC plates, and leaf sections
For additional information about these applications or to learn more about the three iBright model capabilities, visit this page.
To speak with a representative about the iBright imager or request a quote or demo, please fill out this form.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
Most functionality is the same between the standard and secure versions of the iBright Analysis (iBA) software. The primary difference is that the secure version of the software supports the auditing and signature functionality that is needed to support 21CFR laboratories.
To learn more about iBA software, visit this page.
To speak with a representative about the iBright imager or request a quote or demo, please fill out this form.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
iBright Analysis Software (iBA) is available for free download on our website. There is also a Cloud version of iBA software available for users who prefer to work directly from a web browser.
The iBA software is supported on both Windows and Mac computers and can be used by any lab personnel who need it. This software automates data analysis and makes annotation, analysis, and reporting simple. To learn more about the iBA software, view the iBright Analysis Software how-to-videos available on this page.
iBright imagers also support 16 bit .tiff export; this file type can be used with other second party software programs if desired.
To speak with a representative about the iBright imager or request a quote or demo, please fill out this form.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
Once communication is established between the IBright imager and the thermal printer, a print button will become available on the main screen near the image display. Pressing this button will initiate printing of the image currently shown on the instrument screen. This is a separate workflow from the print settings available through the system menu, which are used for printing image reports on a standard network printer.
For more information on iBright features and capabilities, visit this page.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
To establish communication between the Thermal Printer and the iBright imager, first the communication channel on the printer needs to be updated. This can be done by accessing the printer menu, navigating from Digital to Driver and then using the up down toggle to set the printer to channel 897. Once that is complete, the thermal printer can then be connected directly to one of the iBright imager rear USB ports via A to B type USB cable. After installation, the thermal printer can be controlled directly with the iBright onboard software via the on-screen print functionality.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
The iBright System Contrast Tray is included with the purchase of a current iBright Imaging System, inclusive of the CL750, CL1500, and FL1500 iBright Imaging Systems. For additional information on iBright features and capabilities, visit the iBright Imaging Systems page.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
All iBright imager models are compatible with the orange iBright System Contrast Tray (Cat. No. A56597), inclusive of the CL1000, FL1000, CL750, CL1500, and FL1500 iBright imager models. For more information on compatible reagents and applications, visit the iBright Imaging Systems Compatible Reagents page.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
We recommend using the iBright System Contrast Tray (Cat. No. A56597) for imaging all visible protein gel samples such as those stained with Coomassie Blue or Silver Stain. For more information on compatible reagents and applications, visit the iBright Imaging Systems Compatible Reagents page.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
The recommended imaging method depends on the iBright imager model being used. For iBright FL1500 and CL1500 Imaging Systems, we recommend imaging Ponceau-stained samples with the Ponceau application that can be found in Universal mode. For the iBright CL750 Imaging System, we recommend imaging Ponceau-stained samples in Protein gel mode.
We always recommend imaging with the orange iBright System Contrast Tray (Cat. No. A56597), and if needed, adjust the focus or zoom to get bands as sharp as possible. Better quality pictures are obtained when the membrane is wet.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
Channels 3, 4 and 5 on the iBright imager can be used for multiplexing without interference while using the No-Stain Protein Labeling Reagent. Channels 1 and 2 on the iBright imager will show interference. Fluorophores for multiplexing that are compatible with the No-Stain Protein Labeling Reagent are listed here, under the “Secondary Abs” tab (https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/total-protein-normalization.html).
Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.
Smart exposure technology rapidly determines optimal exposure time, which helps minimize the potential for over- or underexposed images, and the need to repeat exposures to get the desired signal. Smart exposure is enabled by built-in algorithms which aim to maximize sensitivity without creating band saturation. Recommended capture times vary depending on the signal intensity of the sample being imaged.
For additional information on iBright functionality, visit the iBright Imaging Systems page.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
The iBright CL1500 Imaging System allows imaging of chemiluminescent western blots and DNA/RNA gels stained with fluorescent nucleic acid stains, and visible protein gels. The iBright FL1500 Imaging System adds the capability of imaging fluorescent western blots using multiple dyes and has greater multiplexing capabilities.
To learn more about the capabilities of the iBright Imaging Systems family of products, please visit the iBright Imaging Systems page.
To speak with a representative about the iBright imager or request a quote or demo, please fill out this form.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.