Zeba™ Dye and Biotin Removal Spin Columns, 5 mL, 25 Columns - FAQs

查看更多产品信息 Zeba™ Dye and Biotin Removal Spin Columns and Filter Plates, 0.5–10 mL - FAQs (A44296S, A44298, A44299, A44300, A44301, A44302, A44303, A44304, A44305, A44296, A44297)

4 个常见问题解答

Are Zeba Dye and Biotin Removal Spin Columns/Filter Plates compatible with mass spectrometry?

Zeba Dye and Biotin Removal Spin Columns/Filter Plates are not directly compatible with mass spectrometry.

Find additional tips, troubleshooting help, and resources within our Protein Dialysis, Desalting, and Concentration Support Center.

Have you tested the Zeba Dye and Biotin Removal Spin Columns/Filter Plates using fixed angle rotor and swinging bucket rotor centrifuges?

The performance of the Zeba Dye and Biotin Removal resin is not affected by the type of centrifuge rotor.

Find additional tips, troubleshooting help, and resources within our Protein Dialysis, Desalting, and Concentration Support Center.

How dilute can my protein sample be for using with Zeba Dye and Biotin Removal Spin Columns/Filter Plates?

Protein samples with as low a concentration as 25 µg/mL can be successfully processed with Zeba Dye and Biotin Removal Spin Columns/Filter Plates to remove dyes, biotin, reducing agents, and crosslinkers and to efficiently recover proteins (>7 kDa).

Find additional tips, troubleshooting help, and resources within our Protein Dialysis, Desalting, and Concentration Support Center.

When should I use Zeba Dye and Biotin Removal Spin Columns/Filter Plates?

Zeba Dye and Biotin Removal Spin Columns/Filter Plates enable fast and efficient removal of non-conjugated fluorescent dyes, biotin, reducing agents, and crosslinkers from protein samples. We have tested removal of the following:
- Alexa Fluor dyes: 488, 555, 647
- Dylight dyes: 488, 550, 650, 650-4x PEG
- Rhodamine dye
- Biotin
- Reducing agents: DTT, TCEP
- Crosslinker: BS3

Find additional tips, troubleshooting help, and resources within our Protein Dialysis, Desalting, and Concentration Support Center.