What is the shelf life for the TMTpro 16plex Label Reagent Set?
The TMTpro 16plex Label Reagent Set is guaranteed for one year from the date of receipt in original, unopened packaging. This information can also be found in the Certificate of Analysis.
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Can the TMT and TMTpro reagents be mixed?
No, the TMT and TMTpro reagents cannot be mixed because the reporter ions are in the same region.
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Can TMTpro label reagents dissolved in anhydrous acetonitrile be dried down and stored for later use?
Yes, unused TMTpro label reagent that has been dissolved in anhydrous acetonitrile can be aliquoted into tubes, dried under vacuum at 25-30 degrees C, purged with nitrogen, and stored in a sealed bag with desiccant at -20 degrees C.
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What size formats is the TMTpro 16plex Label Reagent Set available in?
The TMTpro 16plex Label Reagent Set can be purchased in three different size formats:
1 x 0.5 mg (per tag), Cat No. A44521; sufficient for one 16plex isobaric experiment
6 x 0.5 mg (per tag), Cat No. A44522; sufficient for six 16plex isobaric experiments
1 x 5 mg (per tag), Cat No. A44520; sufficient for ten 16pex isobaric experiments
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At which step should I label my mass spec samples with TMT reagents?
TMT labeling can be performed after reduction, alkylation, digestion, or prior to cleanup, if the sample is buffered at appropriate pH (8-8.5) in a suitable buffer without primary amines (e.g., Tris, glycine). TMT labeling can also be performed after peptide clean-up. Labeling of peptides after clean-up enables measuring and normalizing of the peptide samples for equal mixing.
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