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Oncomine™ BCR IGH SR Assay, RNA - FAQs

查看更多产品信息 Oncomine™ BCR IGH SR Assay, RNA - FAQs (A45484)

5 个常见问题解答

How many libraries can be prepped with each Oncomine BCR IGH SR Assay, RNA?

The assay provides sufficient reagents for rapid preparation of 24 sample libraries.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

What is included in the Oncomine BCR IGH SR Assay, RNA?

The Oncomine BCR IGH SR Assay, RNA consists of the Oncomine BCR IGH-SR Panel, the Ion AmpliSeq Library Kit Plus (Cat. No. 4488990), and the Ion Torrent NGS Reverse Transcription Kit (Cat. No. A45003). Sufficient reagents are provided for the rapid preparation of 24 sample libraries.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

What is the input requirement for the Oncomine BCR IGH SR Assay, RNA?

The assay input requirement ranges from 25 ng to 2 µg DNase-treated total RNA. For RNA samples with a concentration of <6.25 ng/µL, we recommend using the DNAclear Purification Kit (Cat. No. AM1756) to concentrate the sample after performing reverse transcription. We recommend using ≥ 50 ng DNase-treated total RNA depending on sample type, ranging from 50-2,000 ng (see "Saturation analysis of peripheral blood leukocytes (PBL) - Oncomine BCR IGH Assay" on page 159 and "Saturation analysis of tissue - Oncomine BCR IGH Assay" on page 161 of the user guide (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0017438_OncomineTCRBetaAssay_UG.pdf).

When using degraded samples (FFPE) or samples with unknown B or T-cell content, perform sample qualification and quantitation to determine the minimum sample input that is required. For more information, see Appendix B, "Sample qualification and quantitation" on page 130 of the user guide.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

What types of samples is the Oncomine BCR IGH SR Assay, RNA compatible with?

This assay is compatible with isolated B cells, whole blood, peripheral blood leukocytes (PBLs), peripheral blood mononuclear cells (PBMCs), and fresh frozen tissue samples, including FFPE and bone marrow samples.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

What is the Oncomine BCR IGH SR Assay, RNA?

The Oncomine BCR IGH SR Assay, RNA (Cat. No. A45484) is a highly sensitive RNA-based NGS assay that enables the characterization of the immunoglobulin heavy chain complementarity-determining region 3 (CDR3) sequences. The assay accurately measures B cell repertoire diversity and clonal expansion in a wide variety of sample types.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.