Ion Torrent™

Ion AmpliSeq™ Mouse TCR β SR Assay (DNA)

货号: A45488
Ion Torrent™

Ion AmpliSeq™ Mouse TCR β SR Assay (DNA)

货号: A45488
货号
A45488
单位规格
24 reactions
价格(CNY)
货号单位规格价格(CNY)
A4548824 reactions联系我们 ›
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The Ion AmpliSeq Mouse TCR Beta SR Assay is a robust, targeted next-generation sequencing (NGS) assay designed to accurately identify and measure the clonal expansion of T lymphocytes by targeting the complementarity-determining region 3 (CDR3) of the T-cell receptor (TCR) gene locus from gDNA input. The assay can be used for basic and translational research to identify T-cell clones since the nucleotide sequence of the CDR3 region is unique to each T cell and codes for the part of the TCR beta chain that is involved in antigen recognition. For RNA samples, please see Cat. No. A45489.

The high sensitivity and specificity of the assay enables key applications in immunology, immuno-oncology, and vaccine research. The high-sensitivity dual-barcode indexing, flexible input requirements (100 ng–1 μg), high-depth sequencing, and high-throughput capability make this assay ideal for testing a variety of use cases in mouse models, including the role of T cells in generating immune response to immunotherapies such as checkpoint blockade inhibitors, cancer vaccines, or chimeric antigen receptor (CAR) T-cell therapies.

Benefits of the Ion AmpliSeq Mouse TCR Beta SR Assay, DNA, include:
Compatibility with a vast array of research sample types, including fresh-frozen and FFPE tissue, whole blood, peripheral blood leukocytes, and peripheral blood mononuclear cells
High sensitivity and low limit of detection for rare clone identification through dual-barcode indexing
Efficient workflow with 48-hour sample-to-results time
Flexible input requirements ranging from 100 ng to 1 μg
Streamlined and user-friendly informatics solution with multi-sample analysis functionality

The Ion AmpliSeq TCR Beta-SR Mouse Kit, DNA, provides a single pool of multiplex PCR primers and library reagents to generate 90-bp amplicons that can be sequenced on all chip types supported by the Ion GeneStudio S5 sequencing systems, allowing you to pick the best multiplexing configuration for your unique sample batching needs and throughput requirements. The entire workflow from library preparation to analysis of samples can be accomplished in two days using the Ion Chef templating system and the Ion GeneStudio S5 sequencing system.

Learn more about the assay ›

Note: Information about data analysis using Ion Reporter Software v5.12 can be found in the User Guide below.

For Research Use Only. Not for use in diagnostic procedures.

规格

样品类型
DNA
Sequencing Type
基因组和 DNA 测序
适用于(应用)
测序
靶向测序库
反应次数
24
产品线
AmpliSeq™
产品类型
Mouse TCR β SR Assay
运输条件
经验证可置于湿冰或干冰上运输
种属
小鼠
工作流程步骤
文库生成

内容与储存

• Ion AmpliSeq Mouse TCR β-SR A (DNA),储存于冰箱(-5 至 -30℃条件下)中
• Ion AmpliSeq Library Kit Plus,储存于冰箱(-5 至 -30℃)中
• dNTP 预混液,各 25 mM,储存于冰箱(-5 至 -30℃)中

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