PowerTrack™ SYBR Green Master Mix for qPCR, 10 x 5 mL - FAQs

查看更多产品信息 PowerTrack™ SYBR Green Master Mix for qPCR - FAQs (A46113, A46109, A46110, A46112, A46012, A46111)

5 个常见问题解答

What products do you recommend for downstream analysis by qPCR when using the SuperScript IV Single Cell/Low Input cDNA PreAmp Kit?

We recommend using TaqMan Fast Advanced Master Mix for all TaqMan‑based detection methods.

We recommend using PowerTrack SYBR Green Master Mix for all SYBR Green‑based detection methods.

When using the PowerTrack SYBR Green Master Mix, can I use cDNA premixed with yellow sample buffer?

We haven't tested this extensively, but we have tested storage of the cDNA and yellow sample buffer premixed for 2-3 days. For best practice, we recommend keeping the cDNA and yellow sample buffer separate until the reaction set up.

For the PowerTrack SYBR Green Master Mix, there is no description in the quick reference or user guide for UNG incubation time. What is the run time and temperature for the UNG incubation?

The incubation time is not required as UNG is active at room temperature and will degrade amplicons with dUs present.

Is there a specific reason why the PowerTrack SYBR Green Master Mix contains the SYBR Green I dye rather than SYBR GreenER dye?

SYBR Green I dye has been used in PowerTrack SYBR Green Master Mix because our product development team found the SYBR Green I dye to perform more optimally than SYBR GreenER dye during the development of this master mix.

What is the benchtop stability of PowerTrack SYBR Green Master Mix?

Pre-assembled qPCR reactions with PowerTrack SYBR Green Master Mix are stable for up to 8 hrs at room temperature before being run, when stored away from light.