PowerTrack™SYBR Green Master Mix
PowerTrack™SYBR Green Master Mix
Applied Biosystems™

PowerTrack™SYBR Green Master Mix

PowerTrack SYBR Green Master Mix 是一种预先配制、优化的通用型 2X 预混液,可用于实时荧光定量 PCR了解更多信息
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货号数量
A4611210 x 5 mL
A460121 mL
A461095 mL
A461102 x 5 mL
A461115 x 5 mL
A4611350 mL
货号 A46112
价格(CNY)
23,823.00
Each
添加至购物车
数量:
10 x 5 mL
价格(CNY)
23,823.00
Each
添加至购物车
PowerTrack SYBR Green Master Mix 是一种预先配制、优化的通用型 2X 预混液,可用于实时荧光定量 PCR 检测,并提供内置的可视的染料–指示剂,用于精确的反应设置。PowerTrack SYBR Green Master Mix 结合用户提供的引物组和模板,设计用于扩增靶标,以进行准确的基因表达分析。

PowerTrack SYBR Green Master Mix 的功能包括:
•内置的可视指示剂有助于反应设置
•较宽的引物 TM 和浓度兼容性使 qPCR 反应设置具有灵活性,并较大限度减少优化
•在较宽的动态范围内,CTs 的特异性和紧密的重现性’
•带有 UNG 和 dUTP,防止残留 PCR 产物污染

内置可视指示剂
PowerTrack SYBR Green Master Mix 包含一个不会干扰实时荧光定量 PCR 的惰性蓝色染料,可在试管或孔中提供更高的可视性。另提供一种独立的样品缓冲液,其中含有一种惰性黄色染料。选用此黄色样品缓冲液,加样后会得到从蓝色变为绿色的反应混合物,有助于避免移液错误。黄色样品缓冲液有助于反应的设置,但 PowerTrack SYBR Green Master Mix 无需获取卓越的结果。

具有较大的特异性和重现性的配方
PowerTrack SYBR Green Master Mix 包含 Taq DNA 聚合酶,并通过抗体介导的热启动机制严格控制,有助于防止聚合酶在低温下出现不需要的过早活性,从而避免非特异性扩增。PowerTrack SYBR Green Master Mix 旨在为您在较具挑战性的实时荧光定量 PCR 应用中提供卓越的性能。

广泛的仪器兼容性
PowerTrack SYBR Green Master Mix 可在标准或快速循环模式下使用,并且兼容所有 Applied Biosystems 实时荧光定量 PCR 仪器。它还兼容 Bio-Rad CFX96™、CFX384™和iQ™5;Roche LightCycler™480和 Agilent MX3005P™ 系统。为获得较佳结果,引物浓度应在300–800nm的范围内。

用于残留污染控制的 UNG
在常规运行 PCR 的实验室中,针对残留污染的控制是许多研究人员较关心的问题之一,也是假阳性的主要来源。PowerTrack SYBR Green Master Mix 包含 UNG 和 dUTP,能够降解任何之前扩增的 PCR 产物,并有助于防止后续 qPCR 反应的污染。

仅供科研使用。不可用于诊断程序。
规格
适用于(设备)7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, QuantStudio 3, QuantStudio 5, QuantStudio 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Flex, QuantStudio 7 Pro, QuantStudio 12K Flex, Bio-Rad CFX Connect, Bio-Rad CFX384 Touch, Bio-Rad CFX96 Touch, BioRad CFX96, BioRad iCycler iQ, BioRad iQ5, BioRad MyiQ, ViiA 7 System, StepOne System, MJ Chromo4, MJ Opticon, StepOnePlus System, Roche LightCycler 480, Stratagene Mx3000P, Stratagene Mx3005P, Stratagene Mx4000
产品规格管装
包括dUTP, UNG/UDG
Inhibitor Tolerance (Simple)High
多路传输能力Single-plex
反应次数5000 次反应
参比荧光染料ROX(预混)
聚合酶Taq DNA 聚合酶
产品线Applied Biosystems™
产品类型实时荧光定量 PCR SYBR 预混液
数量10 x 5 mL
样品类型DNA
运输条件干冰
足够用于5000 次反应
靶标特异性cDNA or gDNA only
热稳定性8 hr., Assembled Reaction
容量10 x 5 mL
最大浓度2X
检测方法SYBR
适用于(应用)基因表达, 病毒检测
PCR 方法qPCR
反应速度快速,标准
Unit SizeEach
内容与储存
•PowerTrack SYBR Green 预混液
•40 X 黄色样本缓冲液

存储在-20±5°C的黑暗中。

常见问题解答 (FAQ)

What products do you recommend for downstream analysis by qPCR when using the SuperScript IV Single Cell/Low Input cDNA PreAmp Kit?

We recommend using TaqMan Fast Advanced Master Mix for all TaqMan‑based detection methods.

We recommend using PowerTrack SYBR Green Master Mix for all SYBR Green‑based detection methods.

When using the PowerTrack SYBR Green Master Mix, can I use cDNA premixed with yellow sample buffer?

We haven't tested this extensively, but we have tested storage of the cDNA and yellow sample buffer premixed for 2-3 days. For best practice, we recommend keeping the cDNA and yellow sample buffer separate until the reaction set up.

For the PowerTrack SYBR Green Master Mix, there is no description in the quick reference or user guide for UNG incubation time. What is the run time and temperature for the UNG incubation?

The incubation time is not required as UNG is active at room temperature and will degrade amplicons with dUs present.

Is there a specific reason why the PowerTrack SYBR Green Master Mix contains the SYBR Green I dye rather than SYBR GreenER dye?

SYBR Green I dye has been used in PowerTrack SYBR Green Master Mix because our product development team found the SYBR Green I dye to perform more optimally than SYBR GreenER dye during the development of this master mix.

What is the benchtop stability of PowerTrack SYBR Green Master Mix?

Pre-assembled qPCR reactions with PowerTrack SYBR Green Master Mix are stable for up to 8 hrs at room temperature before being run, when stored away from light.