Ion Torrent™

Oncomine™ TCR-β-LR Assay GX

货号: A46297
Ion Torrent™

Oncomine™ TCR-β-LR Assay GX

货号: A46297
货号
A46297
单位规格
32 samples
价格(CNY)
货号单位规格价格(CNY)
A4629732 samples联系我们 ›
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The Oncomine TCR Beta-LR Assay GX is designed to measure T cell diversity and clonal expansion by sequencing T-cell receptor beta chain rearrangements. The assay kit provides single-pool multiplex panel library reagents to perform up to 32 sequencing reactions. The assay is optimized for long-read sequencing on the Ion Torrent Genexus Integrated Sequencer using the Ion Torrent Genexus GX5 Chip. The Genexus Integrated Sequencer performs library preparation, sequencing, analysis, and reporting in an automated workflow that delivers results in a single day. The multi-lane sequencing chip is designed to enable cost-effective processing of libraries.

The assay is designed to efficiently capture all three complementarity-determining regions of the TCR beta chain (CDR1, CDR2, CDR3) with high accuracy. Key applications include predictive or prognostic biomarker discovery, T-cell characterization, and identification of variable gene polymorphisms. The identification of rare and abundant clones can be achieved with a flexible RNA input amount of 10 ng (minimum) up to 1 μg. The use of RNA template allows sequencing of productive and relevant variable (V), diversity (D), and joining (J) rearrangements—which enables the identification of rare clones.

The Oncomine TCR Beta-LR Assay GX supports the following features:
• Long-read RNA sequencing covering the TCR beta chain
• Detection of clones over a wide dynamic range
• Efficient workflow with 24-hr sample-to-result time
• Flexible input requirements of 10 ng to 1 μg RNA
• Compatible with fresh-frozen tissue, whole blood, and sorted cells
• Streamlined and user-friendly informatics solution with multi-sample analysis functionality

Why are immunologists and immuno-oncologists interested in T cell repertoire sequencing research?
• To characterize diversity and monitor features of T lymphocytes in blood and infiltrated tumors
• To identify variable gene polymorphisms implicated in autoimmune disease or immune-mediated adverse events
• To optimize the manufacture and function of therapeutic or engineered T cells

For Research Use Only. Not for use in diagnostic procedures.

规格

样品类型
RNA
Sequencing Type
转录组测序
适用于(设备)
Ion Torrent Genexus 测序仪
适用于(应用)
测序
靶向测序库
产品线
Oncomine™
产品类型
TCR-β-LR Assay GX
运输条件
经验证可置于湿冰或干冰上运输
工作流程步骤
文库生成

内容与储存

• A40282 Oncomine TCR β-LR Assay GX,储存于 -10°C 至 -30°C
• A40254 Ion Torrent Genexus 检测条1,储存于 2–8°C
• A40253 Ion Torrent Genexus 检测条 2-AS,储存于 -10°C 至 -30°C

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