Ion AmpliSeq™ 微生物组健康研究试剂盒

Name: Ion AmpliSeq™ 微生物组健康研究试剂盒
SKU: A46496

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货号	样品数量
A46496	48、256或384
A46495	48、256或384
A46497	48、256或384

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货号 A46496

价格（CNY)

申请报价

样品数量:

48、256或384

本产品具有不同的配置，每个试剂盒可检测48至384份样品，可满足您的微生物组研究需求。该产品包通过完整的分析解决方案为高性价比、全面、靶向性新一代测序 (NGS) 工作流程提供试剂和材料，以对粪便样品中的人肠道微生物组进行高灵敏度、特异性和稳健表征，该解决方案可提供优化的信息学和可视化。

Ion AmpliSeq 微生物组健康研究试剂盒是一种用于表征已与人疾病相关的人肠道微生物组的靶向性 NGS 测定试剂盒。该检测试剂盒含有两个 Ion AmpliSeq 寡核苷酸引物和相关试剂池，用于在 Ion Torrent 平台上生成扩增子文库进行 NGS。该检测试剂盒靶向 16S rRNA 基因中的多个高变区以及 73 个种属特异性序列。通过该试剂盒，使用从人粪便样品中分离出的 DNA 生成用于在 Ion S5 和 Ion Chef 系统中制备模板和测序的文库。

Ion AmpliSeq 微生物组健康研究试剂盒的特点包括：
• 简单—从样品到结果的完整解决方案，包括简单而稳健的工作流程，并包括分析软件
• 稳健—以出色的灵敏度、特异性和重现性对人肠道微生物组进行分析
• 全面—能够针对 16S rRNA 基因的九个 16S 高变区中的八个进行准确的分类学分析和相对丰度估算，并准确检测与人疾病相关的73种关键细菌种属
• 高效—支持在一个 Ion 540 芯片上对多通路检测的32份样品或一个 Ion 550 芯片上的48份样品进行筛选，整个工作流程（包括分析）通常可在三天内完成
• 灵敏—对于 16S rRNA 基因库，兼容低至 1 ng 的 DNA 上样量；对于靶种属库，兼容低至 10 ng 的 DNA 上样量

该测定试剂盒设计用于帮助研究人员理解肠道微生物多样性和组分对人体健康和疾病的影响。这款试剂盒通过测定 16S rRNA 基因中的多个高变区并检测与人疾病（如癌症）相关的73个关键细菌种属（包括与免疫调节药物的潜在功效、胃肠和免疫疾病以及传染病相关的细菌），实现了对微生物组的总体表征。我们设计了菌种特异性引物，使用极佳扩增子技术提供高菌株覆盖率和高特异性性能。使用该方法，可以分析测序产生的读段，以报告样品中微生物的分类学分类和相对丰度，具有高特异性和灵敏度。

仅供科研使用。不可用于诊断程序。

规格

检测方法新一代测序

适用于（应用）测序

样品数量48、256或384

产品线Ion AmpliSeq™

产品类型微生物组健康研究试剂盒

数量256 samples

运输条件干冰

种属人

产品规格试剂盒

Unit SizeEach

内容与储存

• Ion AmpliSeq 微生物组健康研究检测组合，在 -5 至 -30°C 下储存
• Ion AmpliSeq 文库试剂盒 Plus（96次反应），在 -5 至 -30°C 下储存
• IonCode 条形码接头 0101–0196，在 -5 至 -30°C 下储存

货号 A46496 还包含：
• Ion 540 芯片试剂盒，在室温下储存
• Ion S5 Chef 溶液，在室温下储存
• Ion S5 Chef 耗材，在室温下储存

货号 A46497 也包含：
• Ion 550 Chef 试剂，在 -5 至 -30°C 下储存
• Ion S5 测序溶液，在室温下储存
• Ion S5 测序试剂，在 -5 至 -30°C 下储存

常见问题解答 (FAQ)

What is included in the Ion AmpliSeq Microbiome Health Research Kit, Ion 550 bundle?

The Ion AmpliSeq Microbiome Health Research Kit, Ion 550 bundle (Cat. No. A46497) consists of the Ion AmpliSeq Microbiome Health Research - 16S rRNA Gene Pool, the Ion AmpliSeq Microbiome Health Research - Target Species Pool, the Ion AmpliSeq Library Kit Plus, the IonCode Barcode Adapters, the Ion 550 Kit - Chef, and the Ion 550 Chip Kit for the rapid preparation of barcoded sample libraries from bacterial DNA. Sufficient reagents are provided to prepare uniquely barcoded DNA libraries from 384 research samples.
Note: The Ion Library TaqMan Quantitation Kit (Cat. No. 4468802) must be purchased separately.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

What is included in the Ion AmpliSeq Microbiome Health Research Kit, Library bundle?

The Ion AmpliSeq Microbiome Health Research Kit, Library bundle (Cat. No. A46495) consists of the Ion AmpliSeq Microbiome Health Research - 16S rRNA Gene Pool, the Ion AmpliSeq Microbiome Health Research - Target Species Pool, the Ion AmpliSeq Library Kit Plus, and the IonCode Barcode Adapters for the rapid preparation of barcoded sample libraries from bacterial DNA. Sufficient reagents are provided to prepare uniquely barcoded DNA libraries from 48 research samples.
Note: The Ion Library TaqMan Quantitation Kit (Cat. No. 4468802) must be purchased separately.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

Can I use the AmpliSeq_Microbiome_Health_ Analysis plugin for Torrent Suite Software and Ion Reporter Software workflows for my custom 16S or target species AmpliSeq panel?

The Torrent Suite AmpliSeq_Microbiome_Health_ Analysis plugin and Ion Reporter Software workflows were developed and validated exclusively for the Ion AmpliSeq Microbiome Health Research Kit. Open source analysis tools may be available for your custom solutions.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

Can I customize the list of target species for my microbiome diversity research project?

Customization solutions are available through AmpliSeq custom designs (Ampliseq.com), where you can customize your own assays for any species of your interest. For details, please contact your local Field Applications Scientist (FAS).

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

Can the Ion AmpliSeq Microbiome Health Research Kit be used to investigate the human response to SARS-CoV-2 infection?

Several scientific publications have demonstrated the key role played by the gut microbial community in infection susceptibility and severity, including for SARS-CoV-2. The Ion AmpliSeq Microbiome Health Research Kit targets 73 species known to be associated with immune-related diseases, including a large number of known bacterial species with antiviral or immune-modulating properties.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

View all Ion AmpliSeq™ 微生物组健康研究试剂盒，Ion 540 捆绑包 FAQs