Oncomine™ Comprehensive Assay Plus，手动文库制备

The Oncomine Comprehensive Assay Plus is a targeted, next-generation sequencing (NGS) assay that provides a comprehensive genomic profiling solution appropriate for formalin-fixed paraffin-embedded (FFPE) tissues. The assay addresses multiple biomarkers associated with targeted and immune checkpoint therapies, covering over 500 genes, including comprehensive targets that are relevant in cancer. This version of the assay is designed for manual library preparation, not for automated library prepation using the Ion Chef System.

Features of the Oncomine Comprehensive Assay Plus include:
• Enables analysis of variants across 500+ genes
• Detection of SNVs, CNVs, InDels, TMB, MSI, and gene fusions
• Robust performance from as little as 10 ng per pool of nucleic acid isolated from FFPE samples including fine needle biopsies
• Characterized with molecular standards and controls
• Content driven by the Oncomine Knowledgebase and experienced scientists helps assure coverage of key targets aligned to published evidence

The Oncomine Comprehensive Assay Plus provides the reagents for library construction and four pools—two DNA and two RNA—of multiplex PCR primers for preparation of amplicon libraries from FFPE tumor samples. Designed to help you go from hundreds down to a few relevant cancer drivers, this assay is part of a comprehensive workflow that enables NGS data analysis from as little as 10 ng of nucleic acid per pool. The assay is optimized for sequencing on the Ion GeneStudio S5 systems with the Ion 550 Chip to enable up to four samples and a no-template control (NTC) per run. When combined with Oncomine Reporter Software, you can create reports that align labels, guidelines, and clinical trials to your results so you can focus on key drivers of cancer in your research.

From sample to answer
The Oncomine Comprehensive Assay Plus is part of an NGS workflow that helps researchers filter variant results from hundreds to just a few key cancer driver variants. Enabled by Oncomine Informatics, this filtering, as well as the annotations from on-market labels, established guidelines (US-NCCN, US-FDA, EMSO, EMA), and global clinical trials, helps you contextualize and interpret findings to move toward the answers you need.

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