Freedom™ ExpiCHO-S™ Kit - FAQs

View additional product information for Freedom™ ExpiCHO-S™ Kit - FAQs (A46847)

16 product FAQs found

What level of product quality is seen with the Freedom ExpiCHO-S Kit?

After execution and payment for a Commercial Production License, a Cell Line Documentation Package (>200 pages, and specific to the lot of cells purchased), is provided, which includes the entire lineage history of the cells, starting at receipt of the initial vial at Thermo Fisher Scientific through delivery to the purchaser, as well as, all testing reports.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

Do you offer technical support for the Freedom ExpiCHO-S Kit?

Yes. Contact our Field Application Scientists for technical support.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

How can I learn more about commercial licensing of Freedom CHO-S cells?

Please contact our licensing team at outlicensing@thermofisher.com to obtain details.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

Is there a pull-through royalty on final products or an annual maintenance fee when using the Freedom ExpiCHO-S Kit?

No. There is a one-time license fee required prior to IND and an additional fee at the time of commercialization.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

When is a license required for the Freedom ExpiCHO-S Kit?

A commercial use license is required prior to the sale of expressed proteins, or when an IND submission is being prepared for human clinical studies.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

How many transfections are typically required to get high-expressing clones from the Freedom ExpiCHO-S Kit?

Only two transfections are recommended.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

What transfection methods can be used with the Freedom ExpiCHO-S Kit?

FreeStyle MAX Reagent is included in the Freedom ExpiCHO-S Kit as the preferred transfection reagent. ExpiCHO-S Cells (cGMP banked) are also compatible with the Neon Transfection System (optimization of instrument settings is required).

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

What cloning methods can be used with the Freedom ExpiCHO-S Kit?

Limiting dilution cloning is recommended with the Freedom ExpiCHO-S Kit.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

What selection system is used in the Freedom ExpiCHO-S Kit?

Puromycin in combination with methotrexate (MTX) is used to select for stable pools following transfection with the Freedom pCHOm 3.1 and Freedom pCHOp 3.2 vectors.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

What are the main features of the Freedom pCHOm 3.1 and Freedom pCHOp 3.2 vectors?

The Freedom ExpiCHO-S two vector system provides the ability to control the ratio of heavy to light chains while allowing for consistent genomic background for transient and stable workflows.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

Does the Freedom ExpiCHO-S Kit contain documentation for the ExpiCHO-S Cells (cGMP banked)?

A certificate of analysis (COA) demonstrating suitability for research use is available to everyone who purchases the Freedom ExpiCHO-S Kit or the ExpiCHO-S Cells (cGMP banked). A full documentation package, including the cell line's lineage, history, and cGMP testing results, is offered upon execution of a commercial use license.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

What is included in the Freedom ExpiCHO-S Kit?

The Freedom ExpiCHO-S Kit contains all major components needed for the development of stable clones, including ExpiCHO-S Cells (cGMP banked), vectors, and media. A complete list of components, including additional materials required but not included, can be found in the ExpiCHO Expression System Kit manual (https://www.thermofisher.com/document-connect/document-connect.html?url=https%3A%2F%2Fassets.thermofisher.com%2FTFS-Assets%2FLSG%2Fmanuals%2FMAN0025765_FreedomExpiCHO-S_Kit_UG.pdf).

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

Can ExpiCHO-S Cells (cGMP banked) be used for the production of commercial products?

Yes. ExpiCHO-S Cells (cGMP banked) are intended (with the purchase of a commercial license) for the development of biologics. They have been produced in a cGMP animal origin-free environment and have been scaled up in a commercial setting to produce products for clinical testing.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

How successful is the Freedom ExpiCHO-S Kit in creating stable clones?

ExpiCHO-S clones producing a model IgG protein were assessed for stability by comparing volumetric productivity for over 60 generations. Four out of the five clones that were tested maintained expression levels over 60 generations. Expression levels were considered stable if the titer at the end of the stability assessment (at generation 60) was at least 70% of the titer observed at the beginning of the stability study.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

How long does it take to obtain a lead clone with the Freedom ExpiCHO-S Kit?

It is possible to go from transfection to lead clone in as little as 4 months with the Freedom ExpiCHO-S Kit.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

What titers can be achieved with the Freedom ExpiCHO-S Kit?

Volumetric titers of over 3 g/L in fed batch have been obtained with the Freedom ExpiCHO-S Kit.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.