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Ion AmpliSeq™ Microbiome Health Research Assay GX - FAQs

查看更多产品信息 Ion AmpliSeq™ Microbiome Health Research Assay GX - FAQs (A47022)

17 个常见问题解答

What version of Genexus Software is needed to run the Ion AmpliSeq Microbiome Health Research Assay GX kit?

Genexus Software version 6.8 is needed to run the Ion AmpliSeq Microbiome Health Research Assay GX kit.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

What quantitation method do you recommend using for the Ion AmpliSeq Microbiome Health Research Assay GX kit?

We recommend using the Qubit dsDNA Quantification Assay Kits (Cat. No. Q32850, Q32851, Q32853, Q32854).

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

What DNA isolation method do you recommend using for the Ion AmpliSeq Microbiome Health Research Assay GX kit?

We recommend using the MagMAX Microbiome Ultra Nucleic Acid Isolation Kit, with bead tubes (Cat. No. A42358) or MagMAX Microbiome Ultra Nucleic Acid Isolation Kit, with bead plate (Cat. No. A42357) for isolating bacterial DNA from research stool samples.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

Is the GX5 Chip compatible with the Ion AmpliSeq Microbiome Health Research Assay GX kit?

No, only the GX7 Chip has been tested with the Ion AmpliSeq Microbiome Health Research Assay GX kit.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

How many species can be detected with the Ion AmpliSeq Microbiome Health Research Assay GX kit?

The Ion AmpliSeq Microbiome Health Research Assay GX kit can detect 73 species of bacteria.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

How many samples can I run with one Ion AmpliSeq Microbiome Health Research Assay GX kit?

24 samples (12 samples/lane) can be run with the Ion AmpliSeq Microbiome Health Research Assay GX kit.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

Can I use the Genexus Templating Strips 3-GX7 and 4 Kit (Cat. No. A46817) with the GX5 Chip?

No, the Genexus Templating Strips 3-GX7 and 4 Kit (Cat. No. A46817) should be used with the GX7 Chip. Each kit allows for the automated template preparation and chip loading of two GX7 chips using the Genexus Integrated Sequencer.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

What are the storage conditions for the Genexus Templating Strips 3-GX7 and 4 Kit (Cat. No. A46817)?

The Genexus Strip 3-GX7 should be stored at 2-8 degrees C and Genexus Strip 4 should be stored at -30 to -10 degrees C.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

What does the Genexus Templating Strips 3-GX7 and 4 Kit (Cat. No. A46817) consist of?

The Genexus Templating Strips 3-GX7 and 4 Kit (Cat. No. A46817) consists of 8 pairs of 3-GX7 and 4 strips, allowing for up to 2 sequencing runs per kit.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

What Genexus Software version is compatible with the GX7 Chip?

The GX7 Chip is compatible with the Genexus Software version 6.8.0 and later.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

Can the Genexus Coupler be reused?

The Genexus Coupler should not be reused. The chip and coupler should be changed together.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

How long can the GX7 Chip and Genexus Coupler (Cat. No. A46731) remain on the Genexus Integrated Sequencer?

The GX7 Chip and Genexus Coupler (Cat. No. A46731) can remain installed on the instrument until all lanes are used up, or within two weeks of the initial run, whichever comes first.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

What are the storage conditions for the GX7 Chip and Genexus Coupler (Cat. No. A46731)?

We recommend storing GX7 Chip and Genexus Coupler (Cat. No. A46731) at room temperature.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

What does the GX7 Chip and Genexus Coupler (Cat. No. A46731) consist of?

The GX7 Chip and Genexus Coupler (Cat. No. A46731) consists of two GX7 chips and two Genexus couplers, sufficient for up to 8 sequencing runs or two initializations of the Genexus Integrated Sequencer.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

What is the difference between GX5 and GX7 chips?

The GX7 chip provides higher throughput compared to the GX5 chip. The GX5 chip runs 12-15 million reads per lane whereas the GX7 chip runs 20-25 million reads per lane.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

How many reads can the GX7 Chip generate?

The GX7 Chip generates 20-25 million sequencing reads per lane for the 200 bp library, or 80-100 million reads per chip.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

What Genexus software is needed to run GX7 chips?

You need Genexus Software v6.8 or later in order to run GX7 chips.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.