TaqMan™ SCID/SMA 测定试剂盒
TaqMan™ SCID/SMA 测定试剂盒
Applied Biosystems™

TaqMan™ SCID/SMA 测定试剂盒

Applied Biosystems TaqMan SCID/SMA 测定试剂盒是一种多通路实时荧光定量 PCR 测定试剂盒,可检测 SMN1 基因和 TREC了解更多信息
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货号反应次数
A479274,000 次反应
A479288000 次反应
A4792920,000 次反应
货号 A47927
价格(CNY)
-
申请报价
反应次数:
4,000 次反应
Applied Biosystems TaqMan SCID/SMA 测定试剂盒是一种多通路实时荧光定量 PCR 测定试剂盒,可检测 SMN1 基因和 TREC 以及作为内部基因组对照品的 RNase P 基因。如需也能够检测 KREC 的测定试剂盒,请参见 TaqMAN SCID/SMA Plus 测定试剂盒(货号A48566)提供。

TaqMan SCID/SMA 测定试剂盒的特点包括:
• 针对 SCID 和 SMA 进行了优化
• 直接从 DBS 样品中提取,可缩短实操时间并降低成本
• 与96或384次反应方案兼容
• 采用快速循环方案,可在55分钟内获得96份样品的高质量结果
• 分液步骤极少,可轻松实现自动化

从 DBS 到获取结果的完整解决方案
TaqMan SCID/SMA 测定试剂盒可检测 SMN1 和 TREC 以及作为内部基因对照品的 RNase P 基因。此测定解决方案包含每种靶标的引物和探针混合物以及 SMN2 封闭剂。从 DBS 到获取结果,实操时间不到40分钟,且96孔板仅需四个试剂分配步骤。还需一个移液步骤,即从 DBS 裂解物提取板移取至 qPCR 板。检测报告基团染料和惰性参比染料需要一台至少具有五个滤光片的实时荧光定量 PCR 仪器。

TaqMan SCID/SMA 测定试剂盒是一种多通路实时荧光定量 PCR 测定试剂盒,可检测 SMN1 的第7外显子以及 TREC。此测定试剂盒包括作为内部扩增对照的 RNase P 基因,可验证样品提取是否成功。此测定试剂盒针对 SMN1 的高特异性进行了优化,无需交叉检测 SMN2

仅供科研使用。不可用于诊断程序。
规格
适用于(设备)QuantStudio™ 12 k Flex、QuantStudio™ 5、QuantStudio™ 6 Flex、QuantStudio™ 7、QuantStudio™ Dx 系统(RUO 模式)
反应次数4,000 次反应
产品线TaqMan™
数量4,000 reactions
样品类型DNA(基因组)、cDNA
运输条件干冰
检测方法引物-探针
Unit SizeEach
内容与储存
• DNA Extract All Reagents 试剂盒
• TaqPath ProAmp 多通路预混液
• TaqMan SCID/SMA 测定试剂盒

收货后,在 -20°C 下储存。开封后,在 4°C 下储存。

常见问题解答 (FAQ)

How can I order TREC and KREC plasmids directly from GeneArt for use with the TaqMan SCID/SMA Assay and TaqMan SCID/SMA Plus Assay?

Please refer to the following TaqMan SCID/SMA Plus Assay User Guide (pg 6) for instructions on ordering plasmids from GeneArt.

Are the TREC and KREC plasmids included when I purchase the TaqMan SCID/SMA Assay or TaqMan SCID/SMA Plus Assay?

No, you will need to order the TREC and KREC plasmids separately. Please refer to the following TaqMan SCID/SMA Plus Assay User Guide (pg 6) for instructions on ordering plasmids from GeneArt.

Are the TaqMan SCID/SMA Assay and TaqMan SCID/SMA Plus Assay compatible with QuantStudio 6 and 7 Flex Real-Time PCR instruments using QuantStudio Software v1.6.0 (or newer) and firmware v1.0.4 (or newer)?

Yes, the TaqMan SCID/SMA Assay and TaqMan SCID/SMA Plus Assay are compatible with QuantStudio 6 and 7 instruments using QuantStudio Software v1.6.0 (or newer) and firmware v1.0.4 (or newer).

Who is responsible for QuantStudio instrument dye calibration for the TaqMan SCID/SMA Assay and TaqMan SCID/SMA Plus Assay prior to an FAS onsite customer training?

The user is responsible for ordering the required calibration plates prior to the onsite training. If you purchase the onsite training, you may ask the FAS to complete the instrument dye calibration.

What is included when I purchase the Newborn Screening/NBS training (Cat. No. A48619) for the TaqMan SCID/SMA Assay and TaqMan SCID/SMA Plus Assay?

The NBS training (Cat. No. A48619) is a 1 day onsite FAS training which includes hands-on workflow training.