CarrierSeq™ ECS Kit with Ion 540™ Chips (15 samples/chip) - FAQs

View additional product information for CarrierSeq™ ECS Kit with Ion 540™ Chips (15 samples/chip) - FAQs (A48023)

17 product FAQs found

Can I sequence CarrierSeq samples using Ion S5 ExT chemistry?

No, CarrierSeq samples have only been tested using standard Ion S5 sequencing chemistry.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

Can CarrierSeq library preparation be automated using the Ion Chef DL8 kits?

No, CarrierSeq libraries have only been tested for manual library preparation.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

How many pools are present in the Ion AmpliSeq CarrierSeq ECS Panel?

The Ion AmpliSeq CarrierSeq ECS Panel consists of two pools.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

How many amplicons does the Ion AmpliSeq CarrierSeq ECS Panel target?

The Ion AmpliSeq CarrierSeq ECS Panel targets >14,000 amplicons that cover all coding regions and intro/exon boundaries of 420 target genes implicated in 418 inherited disorders.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

Can I run CarrierSeq samples on the Ion 510, Ion 520, or Ion 550 Chips?

No, CarrierSeq samples are only compatible with the Ion 530 and Ion 540 Chips.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

I'm only interested in a subset of the genes covered on the Ion AmpliSeq CarrierSeq ECS panel. Can I construct a library with just those genes?

No, the panel is preconfigured with a set of primers and cannot be altered. However, during analysis in Carrier Reporter Software, you can select which genes you want to report.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

Can I use Ion AmpliSeq HD Library Kit (Cat. No. A37694) to construct CarrierSeq libraries?

No, CarrierSeq library preparation has only been tested with Ion AmpliSeq Library Kit Plus (Cat. No. A35907, 4488990, A38875).

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

Can I use Ion Xpress barcodes with CarrierSeq samples?

No, CarrierSeq samples have only been tested for use with IonCode barcodes.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

Can I run CarrierSeq samples on an Ion PGM or Ion Proton instrument?

No, because the baseline used for analyzing CarrierSeq samples is instrument-specific and chip-specific, CarrierSeq samples cannot be run on an Ion PGM or Ion Proton instrument. They are only compatible with the Ion GeneStudio S5 systems.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

Can I get a local version of Carrier Reporter Software?

Yes, a local version of Carrier Reporter Software can be installed on an Ion Reporter local server. For more information, please contact a Thermo Fisher Scientific representative.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

Is Carrier Reporter Software free to use?

Access to Carrier Reporter Software, Cloud version, is included with the purchase of the Ion AmpliSeq CarrierSeq ECS Panel.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

What software should I use to analyze Ion AmpliSeq CarrierSeq ECS data?

CarrierSeq ECS data can be analyzed using Carrier Reporter Software.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

Can I use the Ion AmpliSeq CarrierSeq ECS Panel to analyze SMN1 and SMN2 , and HBA1 and HBA2?

Yes, difficult-to-characterize target genes due to homology as a result of pseudogenes (GBA and GBAP1 for Gaucher disease research), paralogues (SMN1 and SMN2 for spinal muscular atrophy research), and loci (HBA1 and HBA2 for alpha-thalassemia research) are consolidated in the Ion AmpliSeq CarrierSeq ECS Panel.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

How many genes are covered by the Ion AmpliSeq CarrierSeq ECS Panel?

The Ion AmpliSeq CarrierSeq ECS Panel targets >14,000 amplicons that cover all coding regions and intro/exon boundaries of 420 target genes implicated in 418 inherited disorders, and enables the analysis of 28,000 non-benign ClinVar variants for single-nucleotide variants (SNVs), insertions and deletions (indels), and copy number variants (CNVs) by next-generation sequencing (NGS).

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

Which genes are covered by the Ion AmpliSeq CarrierSeq ECS Panel?

For the complete list of genes on the Ion AmpliSeq CarrierSeq ECS panel, please contact your local Thermo Fisher Scientific representative.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

Can I purchase the Ion AmpliSeq CarrierSeq ECS Panel separately?

Yes, the Ion AmpliSeq CarrierSeq ECS Panel is available for purchase separately (Cat. No. A43471, A48036).

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

How many CarrierSeq samples can I run on one Ion 540 Chip?

For the CarrierSeq ECS Kit with Ion 540 Chips, Cat. No. A43586, we recommend running 16 samples per chip. For the CarrierSeq ECS Kit with Ion 540 Chips, Cat. No. A48023, we recommend running 15 samples per chip.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.