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View additional product information for VeritiPro™ Thermal Cycler, 96-well - FAQs (A48766, A48767, A48768, A48769, A48141, A48770, A47397, A47396, A48771, A47398)
12 product FAQs found
The heated thermal cycler lid is designed to make sure that optimized pressure is applied on the microplate for an efficient PCR reaction. When using PCR tubes, excess pressure on the tubes, from the heated lid can cause deformation of the tubes. To avoid this, we recommend using the Tray/Retainer set, as shown below:
Cat. No. 4381850, MicroAmp 96-Well Tray/Retainer Set for Veriti Systems – This Tray is for use with MicroAmp Strip Tubes (or MicroAmp Reaction Tubes without Caps, 0.2 mL), with the Veriti Thermal Cycler, SimpliAmp Thermal Cycler, and Proflex PCR System.
Cat. No. 403081, MicroAmp 96-Well Tray/Retainer Set – This Tray is for use with MicroAmp Strip Tubes (or MicroAmp Reaction Tubes without Caps, 0.2 mL), with the 7000 System, 2720 Thermal Cycler, and GeneAmp PCR System 9700.
Cat. No. 4379983, MicroAmp 96-Well Tray for VeriFlex Blocks – This Tray is for use with MicroAmp Strip Tubes (or MicroAmp Reaction Tubes with Caps, 0.1 mL), with the Veriti Thermal Cycler, Veriti Fast Thermal Cycler, SimpliAmp Thermal Cycler, and Proflex PCR System.
Please take a look at this video: How to Use Adapter Tray Retainers in Thermal Cycler Starter Kits (https://www.youtube.com/watch?v=1uh2ZxZJtgs).
Find additional tips, troubleshooting help, and resources within our PCR Thermal Cyclers Support Center.
The recommended conditions are determined to ensure no static discharge (low humidity) occurs.
Recommended Conditions:
- Veriti: 20-80% humidity
- VeritiPro: 15-80% humidity
We have not tested Veriti and VeritiPro thermal cyclers in low humidity, below 20%, therefore we cannot recommend using them in those conditions.
Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.
There is no universal ramp rate for a thermal cycler because both the block and sample ramp rates are different for each thermal cycler. The sample ramp rate changes with the volume of the sample since more time and energy are required to heat higher sample volumes. The ramp rate may also need to be changed for a particular PCR assay or primer pair if you are trying to use the same PCR assay on two different thermal cyclers, for example.
As a reference, the block ramp rate commonly used with DreamTaq Polymerase on the MiniAmp thermal cycler is 3.0 C/sec. In a 50 µL PCR reaction, this translates to a sample ramp rate of ~1 C/sec.
Please refer to the following application note for further information about thermocycler ramp rates: https://assets.thermofisher.com/TFS-Assets/LSG/brochures/Thermal_Cycler_Ramp_RatesAppNote.pdf
Find additional tips, troubleshooting help, and resources within ourPCR Thermal Cyclers Support Center.
The VeritiPro Thermal Cycler can be connected wirelessly with an optional High-Power USB W-Fi Module, Cat. No. A26774.
To add an infinite hold on the VeritiPro Thermal Cycler, select the infinity symbol for program.
The VeritiPro Thermal Cycler features VeriFlex blocks for enhanced PCR functionality. Six separate Peltier blocks (6 columns each) provide maximal versatility and flexibility and offer two key benefits:
- PCR optimization: Each block can be set with a specific temperature, which is ideal for precise control over PCR optimization.
- Run more experiments: The three Peltier blocks can also be used to run up to six different annealing temperatures in the same run.
The AutoDelta feature allows you to incrementally increase or decrease the temperature or hold time with each cycle for a given step within a cycling stage. One common application for this is touchdown PCR.
The maximum block ramp rate for the VeritiPro Thermal Cycler is 6.0 degrees C/sec.
The compatible plates and plastics are the same as those used with the ProFlex 96-Well PCR System and can be viewed here: https://www.thermofisher.com/us/en/home/life-science/pcr/pcr-plastics/thermal-cycler-plastics/proflex-96-3x32.html.
We currently offer only a 96-well, 0.2 mL (standard) VeriFlex block that is not interchangeable.
The Thermal Cycler Fleet Control Software allows monitoring of the following instruments from within a firewall:
SimpliAmp Thermal Cycler
ProFlex PCR System
Veriti Thermal Cycler
VeritiPro Thermal Cycler
Automated Thermal Cycler (ATC)
The Thermal Cycler Fleet Control Software is compatible with the following instruments:
- SimpliAmp Thermal Cycler
- ProFlex PCR System
- Veriti Thermal Cycler
- VeritiPro Thermal Cycler
- Automated Thermal Cycler (ATC)
- MiniAmp Thermal Cycler
- MiniAmp Plus Thermal Cycler