MagMAX™ 病毒/病原体核酸分离试剂盒
Invitrogen17万+抗体限时买二赠一,靶点广,灵活用!
Invitrogen17万+抗体限时买二赠一,靶点广,灵活用!
MagMAX™ 病毒/病原体核酸分离试剂盒
Applied Biosystems™

MagMAX™ 病毒/病原体核酸分离试剂盒

MagMAX 病毒/病原体核酸分离试剂盒旨在从病毒和革兰氏阴性菌样品(如血液、拭子、尿液和病毒运送培养基 (VTM))中回收 RNA 和 DNA了解更多信息
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货号数量
A48310Up to 1000 Preps
A42352Up to 100 Preps
货号 A48310
价格(CNY)
26,826.00
Each
添加至购物车
数量:
Up to 1000 Preps
价格(CNY)
26,826.00
Each
添加至购物车
MagMAX 病毒/病原体核酸分离试剂盒旨在从病毒和革兰氏阴性菌样品(如血液、拭子、尿液和病毒运送培养基 (VTM))中回收 RNA 和 DNA。该试剂盒采用 MagMAX 磁珠技术,确保可重复回收与多种应用(包括实时荧光定量 PCR、数字 PCR 和二代测序)兼容的高质量核酸。

MagMAX 病毒/病原体核酸分离试剂盒的特点包括:
•为 KingFisher Flex 和 KingFisher Duo Prime 系统设计的自动化预制方案
• 快速程序、可在 <30 分钟内处理 96 份样品
•灵活的方案可容纳 200 μL 至 2 mL 生物液体或转移培养基的样品量输入
•无需载体 RNA
•洗脱体积范围为 50 至 100 μL

96 孔深孔或 24 孔深孔板形式的较大样品输入
MagMAX 病毒/病原体试剂盒中包括的优化试剂可实现较大的样品输入量,从而增加 RNA 和/或 DNA 的回收量。在 KingFisher 96 孔深孔板中,每孔可处理多达 400 μL 的样品。对于更大的样品量,可采用 KingFisher 24 孔深孔板,每孔可处理多达 2 mL 样品。

MagMAX 磁珠法纯化的优势
与其他分离核酸的技术相比,磁珠具备许多优势。与玻璃纤维过滤柱相比,磁珠可更高效地结合核酸,从而获得更高且更一致的产量。此外,由于未使用过滤柱和真空歧管,不存在样品中的细胞颗粒堵塞过滤柱的风险。这种堵塞问题对于通常用于病毒检测的富含蛋白的大量样品(如全血或血浆)特别重要。

MagMAX 病毒/病原体总核酸分离试剂盒中的 Dynabeads MyOne Silane 具有经过优化的类硅胶表面化学结构和高比表面积,可实现高效动力学和高灵敏度核酸捕获。上述特性使 Dynabeads MyOne Silane 成为回收体液中低浓度病毒 RNA 和 DNA 的理想选择。一旦捕获,这些核酸即可在 50 μL 洗脱缓冲液中洗脱,用于下游应用。

仅供研究使用。不可用于诊断程序。
规格
产品线MagMAX™
纯化时间30 min。
数量Up to 1000 Preps
运输条件室温
产品规格96孔深孔板, 24孔深孔板
Unit SizeEach
内容与储存
•550 mL 结合溶液(在室温下储存)
•1000 mL 洗涤液(在室温下储存)
•100 mL 洗脱溶液(在室温下储存)
•10 mL 蛋白酶 K(在室温下储存)
•20 mL DNA/RNA 结合珠(在室温下储存)

常见问题解答 (FAQ)

I accidentally stored my MagMAX Viral/Pathogen Nucleic Acid Isolation Kit at room temperature for several months. The beads are supposed to be stored at 4 degrees C. Are they still usable?

Yes, the kit is still usable.

I am getting MagMAX bead carryover in some wells of my plate. What might be causing this and how can I resolve it?

Some bead carryover is common and has not been documented to affect downstream assays. The most common cause of bead carryover is too much input sample. The best way to determine if the sample amount is the cause of the issue is to run PBS with Xeno (artificial DNA/RNA) in the lysis buffer and then check if there is still bead carryover. If that is not the cause, the initial sample may require special processing, such as bead beating or liquification. If there is still bead carryover without the original sample, and in particular, if the carryover is present in the same wells for multiple runs, then this may be an instrument alignment issue.

Do I need any additional consumables when using MagMAX kits on the MagMAX Express-96 instrument or a KingFisher instrument?

Plastic consumables need to be purchased separately in order to use the MagMAX reagents on the MagMAX Express-96 or a KingFisher instrument. The particular consumables and the amount required will vary by kit and protocol so please check the user guide for the particular MagMAX kit to be sure that all required plastic consumables are available prior to sample processing.

Where can I find Bindlt script protocol (.bdz) files for use of MagMAX kits with the KingFisher Flex Purification System and KingFisher Duo Prime Purification System?

The best place to download KingFisher Flex and KingFisher Duo Prime protocols for a particular MagMAX kit is the relevant MagMAX kit product page. The Bindlt script protocol (.bdz) files can be found under the Documents>Product literature section.

Can I scale up the MagMAX kit reaction volumes? If so, how should I do this?

Yes, reaction volumes for MagMAX kits can be scaled up. In general, the amount of MagMAX reagents should be scaled up proportionally for the increase in sample input amount. Please see the specific kit user guide for any recommendation. If none are included, then optimization may be necessary.