StemScale™ PSC Suspension Medium
StemScale™ PSC Suspension Medium
Gibco™

StemScale™ PSC Suspension Medium

Gibco StemScale PSC Suspension Medium supports the growth of pluripotent stem cells (PSCs) in suspension through the self-assembly of spheroids. The unique formulation of StemScale medium provides superior expansion while maintaining high viability and consistent spheroid formation across multiple PSC lines.
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Catalog NumberQuantity
A49650011 L
Catalog number A4965001
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7,414.80
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Ends: 31-Dec-2025
9,399.00
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Quantity:
1 L
Price (CNY)
7,414.80
Online Exclusive
Ends: 31-Dec-2025
9,399.00
Save 1,984.20 (21%)
Each
Add to cart

Gibco StemScale PSC Suspension Medium supports the growth of pluripotent stem cells (PSCs) in suspension through the self-assembly of spheroids. The unique formulation of StemScale medium provides superior expansion while maintaining high viability and consistent spheroid formation across multiple PSC lines. Simplified medium exchange and passaging workflows offer scalability in different formats and flexibility in feeding schedule. Pluripotent stem cells expanded in StemScale medium maintain pluripotency and normal karyotype across multiple passages and can be differentiated to the three germ layers.

Maximal expansion potential

Pluripotent stem cells cultured in StemScale PSC Suspension Medium consistently achieve 5-10 fold expansion per passage and three times the expansion capability versus other PSC suspension media. Long term expansion of PSCs in StemScale medium (>30 passages) can be accomplished without loss of expansion potential, pluripotency, or genomic stability.

Superior workflow

StemScale PSC Suspension Medium promotes the highly efficient self-aggregation of PSCs into spheroids, eliminating the need for microcarriers. Unlike other self-assembly methods that require batch feeding, PSCs maintained in StemScale medium are fed by a 50% medium replacement strategy. This method reduces metabolic waste in the culture while maintaining constant media volumes, important for maintenance of spheroid size. The use of cell strainers during passaging, which can lead to cell loss, is not required and contributes to the ability to scale into larger vessel sizes for workflow scale-up.

Compatible with multiple vessel types

Pluripotent stem cells can be expanded in StemScale medium across multiple vessel types, including 6-well plates, shaker flasks, and small-scale bioreactors, allowing users to scale PSC production as needed without sacrificing performance.

Flexible feeding schedule

StemScale medium offers a flexible workflow that allows users to selectively determine when to passage cultures. Feeding days can be skipped, providing the option of a weekend-free passaging schedule.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell LineEmbryonic Stem Cells (ESCs), Induced Pluripotent Stem Cells (iPSCs)
Cell TypeEmbryonic Stem Cells (ESCs), Induced Pluripotent Stem Cells (iPSCs)
ConcentrationStemScale Basal Medium (1X); StemScale Supplement (10X)
Culture Environment36°C to 38°C, humidified, 5% CO2, on CO2-resistant shaker platform
Manufacturing QualitycGMP for medical devices, 21 CFR Part 820 and ISO 13485
Product TypeStem Cell Media
Quantity1 L
Shelf Life12 Months From Date of Manufacture
ClassificationAnimal Origin
Culture TypeSuspension Cell Culture, Stem Cell (Human, iPS - Induced Pluripotent Stem, Embryonic)
FormLiquid
Serum LevelSerum-free
SterilitySterile-filtered
Sterilization MethodSterile-filtered
With AdditivesPhenol Red
Unit SizeEach
Contents & Storage
• 900 mL Basal Medium, store at 2–8°C and protect from light
• 100 mL Supplement, store at -20°C and protect from light

Frequently asked questions (FAQs)

How does the StemScale PSC Suspension Medium passaging protocol compare to protocols for other suspension culture media?

The StemScale StemScale PSC Suspension Medium passaging protocol recommends passaging every 4-5 days, depending on spheroid size. We recommend passaging suspension cultures when the average spheroid diameter is 400 µm, which occurs approximately after 4-5 days of growth. Passaging cultures on day 3 is also an option but may result in a lower cell yield. Day 3 passaging can be performed to avoid passaging on a weekend.

Passaging protocols for other suspension culture media may offer less flexibility, depending on whether they utilize a fed-batch or overlay strategy. Generally, these cultures require a strict passaging schedule with little room for flexibility on the weekend.

The StemScale PSC Suspension Medium passaging protocol does not require the use of microcarriers or cell strainers, while other PSC suspension culture medium protocols do

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Are there advantages to the StemScale PSC Suspension Medium feeding method as compared to those for other suspension culture media?

The StemScale PSC Suspension Medium feeding method involves replacing 50% of the spent medium with fresh medium. This feeding method prevents spheroids from being cultured in a medium that is accumulating significant quantities of waste by-products.

Feeding methods that use a fed-batch or overlay strategy do not remove the spent medium from suspension cultures. This method reduces hands-on time when feeding cultures; however, the accumulation of waste by-products is likely to negatively impact the health of these cells.

The StemScale PSC Suspension Medium feeding strategy also ensures a consistent volume through the growth period of the PSC spheroids. This is in contrast to batch-feeding methods where the volume increases incrementally. Since culture volume is one of the determinants of shear force and spheroid size, the 50% feeding strategy maintains this variable constant throughout the growth period.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How does the StemScale PSC Suspension Medium feeding schedule differ from that of other suspension culture media?

While other suspension culture media may require daily feeding of suspension cultures, the StemScale feeding schedule allows for every-other-day feeding. With StemScale PSC Suspension Medium, cultures can be fed daily to maximize cell health. However, users have the option to feed every other day without sacrificing performance while also providing more flexibility in the suspension culture schedule.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Managing multiple components in a medium kit can be challenging. Does the StemScale PSC Suspension Medium kit have a similar number of components as others?

The StemScale PSC Suspension Medium kit consists of two components: a basal medium and a supplement. Following reconstitution, the complete medium is used for all steps, from initiating cultures to feeding cultures. Other suspension culture medium kits sometimes consist of multiple components, which may be utilized at different points in the suspension culture process.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

I'm using StemScale PSC Suspension Medium. What do the suspension cultures look like if I used TrypLE Select Enzyme without including DNase?

Without DNase, the presence of small quantities of lysed DNA due to dissociation by TrypLE Select Enzyme will cause some spheroids to aggregate into a single clump.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

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