Genexus™ Templating Strips 3B-GX5 and 4

The Ion Torrent Genexus Templating Strips 3B-GX5 and 4 kit provides eight each of the strips required to perform automatedRead more

Catalog Number	Quantity
A49782	2 runs kit

Catalog number A49782

Price (CNY)

Quantity:

2 runs kit

The Ion Torrent Genexus Templating Strips 3B-GX5 and 4 kit provides eight each of the strips required to perform automated template preparation and chip loading of two GX5 chips using the Genexus Integrated Sequencer. This kit is specifically for use with the Oncomine Myeloid GX v2 assay. For all other Genexus assays, please use the Genexus Templating Strips 3-GX5 and 4.

This kit, combined with the GX5 Chip and Genexus Coupler, supports flexible, fully automated template preparation and chip loading of libraries prepared using the Genexus Integrated Sequencer or with an alternative protocol.

As part of the Genexus Integrated Sequencer workflow, the Genexus Templating Strips 3B-GX5 and 4:
• Automate template preparation and GX5 Chip loading for Oncomine Myeloid GX v2 assays
• Support batching of clinical research samples in a cost-effective manner
• Maximize flexibility with the ability to load one to four chip lanes per run
• Deliver fast and simple instrument setup, with less than five minutes of hands-on time

The Genexus Integrated Sequencer automates library preparation, sequencing, analysis, and reporting in a hands-off, walkaway workflow to deliver results in a single day. The innovative, multilane sequencing chip is designed to enable cost-effective processing of 1 to 32 single pool libraries.

Specifications

For Use With (Application)Sequencing

For Use With (Equipment)Genexus™ Integrated Sequencer

No. of Reactions2 Runs

Quantity2 runs kit

Sample TypeDNA and RNA from Blood or Bone Marrow

Unit SizeEach

Frequently asked questions (FAQs)

What do I need to do to prep Genexus Templating Strips 3B-GX5 and 4-GX5 (Cat. No. A49782) for use on the Genexus platform?

Remove the templating strips from their boxes in the refrigerator or freezer and get them ready them for loading in the sequencer by equilibrating to/thawing at room temperature for 30 mins. Confirm that the strip contents are completely thawed before loading in the sequencer. If you are delayed in loading, keep the thawed strips on ice or at 4 degrees C until you load them in the sequencer.

Genexus Strip 3B‑GX5 contain magnetic beads in one or two positions, yellow or brown in color, that sometimes get trapped in the upper “keyhole” of the tube. Dislodge these beads from the keyhole before installing the strip in the sequencer using the following procedure for each strip:
- Invert the strip 3-4 times to dislodge beads that are trapped in the keyholes.
- To remove any remaining beads and liquid from the keyholes, grasp the strip at one end with the strip seal facing up, then swing the strip with a rapid, downward centrifugal arm motion, ending with a sharp wrist-flick.
- Grasp the strip at the other end, then repeat the centrifugal motion.
- Check tube positions for significant amounts of beads that are still trapped in keyholes and then repeat the centrifugal motion, if needed. It is acceptable if a few beads remain in the keyhole or on the tube wall, but most should be either in suspension or in a pellet at the bottom of the tube.

What is the difference between Genexus Templating Strips 3B-GX5 and 4-GX5 (Cat. No. A49782) and Genexus Templating Strips 3-GX5 and 4 (Cat. No. A40263)?

Genexus Templating Strips 3B-GX5 and 4-GX5 (Cat. No. A49782) has new Genexus Strip 3B that has the chemistry modified to improve templating and sequencing of GC rich regions.

What application is Genexus Templating Strips 3B-GX5 and 4-GX5 (Cat. No. A49782) compatible with?

Genexus Templating Strips 3B-GX5 and 4-GX5 is specifically designed for use with the Oncomine Myeloid GX v2 assay. For all other Genexus assays, please use Genexus Templating Strips 3-GX5 and 4 (Cat. No. A40263).

What are the components of Genexus Templating Strips 3B-GX5 and 4-GX5 (Cat. No. A49782)?

Genexus Templating Strips 3B-GX5 and 4-GX5 provides 8 pairs of strips per kit - Genexus Templating Strips 3B-GX5 (Part No. A47712) (Orange, 8 strips) and Genexus Templating Strips 4-GX5 (Part No. A46816) (Yellow, 8 strips). Each pair of strips is required to perform automated template preparation and chip loading of two GX5 chips using the Genexus Integrated Sequencer.

What are the shipping and storage conditions for Genexus Templating Strips 3B-GX5 and 4-GX5 (Cat. No. A49782)?

Genexus Templating Strips 3B-GX5 (Part No. A47712) (Orange, 8 strips) ships on a gel pack and should be stored at 2 to 8 degrees C. Genexus Templating Strips 4-GX5 (Part No. A46816) (Yellow, 8 strips) ships on dry ice and should be stored at -30 to -10 degrees C.