TaqMan™ 微生物检测测定试剂
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Applied Biosystems™

TaqMan™ 微生物检测测定试剂

Applied Biosystems TaqMan 微生物检测和 TaqMan 抗生素耐药性测定试剂用于微生物物种和抗生素耐受性基因的实时荧光定量 PCR 检测。这些测定试剂包括一对未标记的 PCR了解更多信息
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货号数量
A50137250 μL
货号 A50137
价格(CNY)
2,283.00
Each
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数量:
250 μL
Applied Biosystems TaqMan 微生物检测和 TaqMan 抗生素耐药性测定试剂用于微生物物种和抗生素耐受性基因的实时荧光定量 PCR 检测。这些测定试剂包括一对未标记的 PCR 引物和一根 TaqMan 探针,探针的 5’ 端带有染料标记 (FAM),3’ 端带有小沟结合物 (MGB) 和非荧光淬灭剂 (NFQ)。

Applied Biosystems TaqMan 微生物检测和 TaqMan 抗生素耐受性测定试剂的特点包括:
易用 — 只需加入 DNA 和预混液并运行 qPCR — 无需熔解曲线
特异性 — 测定试剂采用专有的含 MGB 的探针,这种探针比非 MGB 探针少了多达15个碱基,从而提高了测定试剂的特异性
灵敏 — TaqMan 测定试剂是测量低丰度微生物靶标的理想选择
准确 — 可以较高的定量准确度识别微生物靶标
丰富的内容物 — 现有300多种预设计微生物检测测定试剂和100多种预设计抗生素耐受性测定试剂
黄金标准 TaqMan qPCR 化学试剂 — TaqMan 测定试剂利用赛默飞世尔科技的生物信息学测定试剂设计流程,以帮助确保高特异性和超低的交叉反应性—即使对于高度相关的微生物种属和亚类
快速交付 — 测定试剂在 1–2 天内即可发货(见下文)

大约发货时间
• 现货库存产品:北美地区 1–2 天*
• 按订单生产产品:北美地区 4–6 天*

我们所有的预设计 TaqMan 基因表达测定试剂均享有 TaqMan 测定试剂 qPCR 保证。**

*我们较受欢迎的测定试剂在北美地区都有库存,如果条件允许,可次日交货。如果测定试剂缺货,可能需要多等 3–5 个工作日才能交货。

**受限于条款和条件。有关完整的详细信息,请访问 www.thermofisher.cn/taqmanguarantee

规格
3' 引物修改FAM
5' 引物修改MGB-NFQ
产品规格管装
反应次数250 次反应
产品线TaqMan
数量250 μL
运输条件室温
最大浓度20X
检测方法引物-探针
适用于(应用)病原检测
Unit SizeEach
内容与储存
存储在 -15°C 到 -25°C。

常见问题解答 (FAQ)

What are the concentrations of the primers and probes for TaqMan microbial assays?

Our predesigned and custom TaqMan microbial 20X assays have a primer concentration of 900 nM and a probe concentration of 250 nM in the final 1X concentration.

Find additional tips, troubleshooting help, and resources within our TaqMan Primers and Probes Support Center.

For TaqMan Microbe Detection and Antibiotic Resistance Assays, what do the different banners mean (Qualified, Recommended, Control)?

- A recommended assay is the assay with the best strain coverage based on in silico prediction. In other words, recommended assays have the highest number of predicted on-targets and lowest number of predicted off-targets.
- A qualified assay has gone through R&D testing within the context of our main microbe detection applications, such as urinary tract, respiratory tract, and vaginal microbiota detection.
- A control banner is used exclusively for common control assays used in microbe detection applications.

I would like to order a control for my TaqMan Microbe Detection and Antibiotic Resistance Assay. Do you offer one? Do you have options for a custom control?

We have several controls for our pathogen-related content. To find a list of all available amplification controls, simply search in the Microbe Detection Search Tool (https://www.thermofisher.com/microbe-detection/taqman/query/) and click on the “Controls” option. Please contact Technical Support if you are unsure whether a particular control is compatible with your assay of interest. We also have options for creating a custom control, containing your specific targets of interest. You can find more information here: https://www.thermofisher.com/us/en/home/clinical/clinical-genomics/pathogen-detection-solutions/real-time-pcr-panel-controls.html

What protocol do you recommend using for the TaqMan Microbe Detection and Antibiotic Resistance Assays?

When using the assays in a single-tube format, we recommend following this general protocol (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0018536_TaqManMicrobialAssays_SingleTube_QR.pdf).

How do I find the TaqMan Microbe Detection and Antibiotic Resistance Assay I'm looking for and search for multiple targets?

There are several ways to search for TaqMan Microbe Detection and Antibiotic Resistance Assays. From the main Thermo Fisher Scientific website page (www.thermofisher.com), hover over the word “Popular” in the top navigation bar. From the menu that comes up, select “TaqMan Real-Time PCR Assays”. Under “The right TaqMan Assay is just a couple of clicks away”, from the TaqMan Assay dropdown, select “Microbe & ABR Detection”. In the search box, you can then enter your target organism and find your assay of interest.

You can also use these friendly URLs:
https://www.thermofisher.com/microbe-detection/taqman/query/
https://www.thermofisher.com/antibiotic-resistance/taqman/query/

To search for multiple targets, from the search results displayed above, click on the link in the upper right for “Search Multiple Targets”.