Countess™ 3 FL Automated Cell Counter
Experience nuclei counting with fluorescent-mode counting algorithms, simplified workflows, built-in dilution calculators and reports using the latest version of Countess software with optional 21 CFR Part 11 compliance. Download now!
Countess™ 3 FL Automated Cell Counter
Invitrogen™

Countess™ 3 FL Automated Cell Counter

Experience rapid cell counting with the Countess 3 FL Automated Cell Counter. This brightfield and fluorescence cell counter has advanced optics, automated focus and lighting, and AI-based image analysis software for counting cells and nuclei, monitoring fluorescence, and measuring cell viability.
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货号类型质保期软件
A50303用户安装型自动细胞计数器AB Repair Center (ABRC) Extended WarrantyCountess™ 3/3 FL Cell Counter Software
A49866自动细胞计数器及附件StandardCountess™ 3/3 FL Cell Counter Software
A49892用户安装型自动细胞计数器Rapid Exchange (REX) Extended WarrantyCountess™ 3/3 FL Cell Counter Software
A49893用户安装型自动细胞计数器AB Repair Center (ABRC) Extended WarrantyCountess™ 3/3 FL Cell Counter Software
A50299用户安装型自动细胞计数器Rapid Exchange (REX) Extended WarrantyCountess™ 3/3 FL Cell Counter Software
AMQAF2000全自动细胞计数器StandardCountess™ 3/3 FL Cell Counter Software
AMQAF2001Automated Cell CounterStandardCountess™ 3/3 FL Cell Counter Software with 21 CFR Part 11 Software Module
7 选项
货号 A50303
价格(CNY)/ 1 each
-
类型:
用户安装型自动细胞计数器
质保期:
AB Repair Center (ABRC) Extended Warranty
软件:
Countess™ 3/3 FL Cell Counter Software
The Countess 3 FL Automated Cell Counter is a benchtop brightfield and fluorescence cell counter with advanced optics, automated focus and lighting, and image analysis software driven by a neural network-based machine-learning algorithm for rapid automated cell counting of primary and immortalized cell lines. The Countess 3 FL counter allows brightfield or fluorescence cell counting with three-channel flexibility (brightfield and two optional fluorescence channels) enabling researchers to count cells and isolated nuclei, monitor fluorescence, examine apoptosis, and measure cell viability.

Features of the Countess 3 FL Automated Cell Counter include:

  • Accurate—highly accurate machine-learning algorithm applied to automated cell counting, eliminates the subjectivity of manual cell counting and user-to-user variability
  • Flexible—accepts up to two EVOS™ light cubes, user selectable from fifteen fluorescent channels; compatible with both disposable and reusable cell counting chamber slides
  • Fast—counts live and dead cells, measures viability, reports average cell size, calculates percent cell aggregates, and saves data with a single touch, in about 20 seconds
  • Convenient—requires no cleaning, routine maintenance, or calibration; high-resolution capacitive touchscreen and simple user interface provide quick startup and requires minimal training
  • Versatile—perform automated counting of single cells and isolated nuclei for RNA sequencing and gene expression applications in single-cell analysis workflows

The Countess 3 FL Automated Cell Counter works in two simple steps:

  • Mix 10 μL of sample with 10 μL of trypan blue or other desired reagent, including ReadyCount™ stain, and pipet into a disposable or reusable Countess chamber slide.
  • Insert the slide into the instrument. Rapid Capture and Auto-Save modes allow automated cell counting with a single touch. Focus, lighting, and file saving are executed automatically.

Obtain accurate cell counts

The Countess 3 FL Automated Cell Counter uses trypan blue staining combined with auto-focus and lighting driven by a machine-learning image analysis algorithm to obtain accurate cell and viability counts. The measurement range extends from 1 x 104 to 1 x 107 cells/mL (the optimal range is 1 x 105 to 4 x 106 cells/mL), which is broader than that of a hemocytometer.

Simple operation with fluorescent application versatility

Operated using an intuitive user interface, automated cell counting with the Countess 3 FL counter gives you the data you need to prepare your sample for the next passage or experiment and allows you to multiplex with a variety of fluorescent dyes and proteins excited and detected via EVOS™ light cubes. With both brightfield and fluorescence cell counting options, you can perform cell counts for routine cell culture applications along with more extensive fluorescence analysis of cells such as live vs. dead, nucleated vs. non-nucleated, and transfected vs. untransfected. You no longer need complex, expensive instruments to obtain reliable data.

Transfer data and images to your PC or cloud

Using the USB port on the Countess 3 FL Automated Cell Counter and any USB drive, you can save your cell count results and images and transfer them to your PC. For your convenience, a USB drive and WiFi dongle are supplied with the Countess 3 FL instrument.

Improve downstream single-cell analysis

Ensure accurate single-cell analysis and RNA sequencing results by minimizing cell aggregates and obtaining precise viable cell counts. For optimal single-cell data output, use the percent cell aggregates on the Countess 3 FL to confirm <5% aggregates in the starting sample before single-cell analysis. Staining with fluorescent dyes such as the ReadyCount stains and using the Countess 3 FL-based count mode offers more accurate counts and viability measurements for samples with debris or out-of-focus cells in brightfield. The Countess 3 FL and ReadyCount stains are also recommended for counting isolated nuclei in scATAC-Seq studies. View the single cell and nuclei counting application note for more details.

The Countess 3 FL Automated Cell Counter can be purchased as the instrument (Cat. No. AMQAF2000) or a variety of packages bundled with additional accessories, extended warranties, or software:

  • Countess 3 FL Starter Package (Cat. No. A49866) includes:
    • Countess FL Automated Cell Counter
    • EVOS™ GFP & RFP light cubes
    • Reusable slide
    • 500 disposable slides with trypan blue

Extended warranty instrument and starter packages

The Countess 3 FL Automated Cell Counter comes with a standard 1-year warranty. Keep your cell counter up and running with the addition of a 3-year extended warranty that covers all costs for shipping, parts, and labor for repairs. Countess 3 FL instrument and Starter Packages are available with two different types of extended warranty packages:

  • Rapid Exchange (REX) Extended Warranty (3 years)—replacement shipped out in 1 business day for labs with critical timelines
    • Available with the instrument only (Cat. No. A49892) and the Countess 3 FL Starter Package (Cat. No. A50299)
  • AB Repair Center (ABRC) Extended Warranty (3 years)—instrument repaired and returned in ~3 weeks for regulated labs required to maintain asset tagging consistency (loaner instrument shipped within 1 business day with upgrade to an ABRC Support Plus contract)
    • Available with the instrument only (Cat. No. A49893) and the Countess 3 FL Starter Package (Cat. No. A50303)

Countess 3 FL with 21 CFR Part 11 Software Module (Cat. No. AMQAF2001)

The included Countess 3 21 CFR Part 11 Software Module is for use with the Countess 3 and Countess 3 FL automated cell counters and supports compliance with 21 CFR Part 11 FDA guidelines for security, auditing, and electronic (SAE) signatures using an SAE administration console. The 21 CFR Part 11 software can also be purchased separately (Cat. No. A51025).

For Research Use Only. Not for use in diagnostic procedures.
规格
类型用户安装型自动细胞计数器
产品线Countess™
相机5 MP
适用于(设备)台盼蓝、Countess 腔室玻片和 ReadyCount 染色剂
检测方法亮场 & 荧光
光源亮场 & EVOS 光立方
放大2.5X
光学3 Channels (Brightfield and 2 slots for EVOS™ LED light cubes)
显示器LCD 1280 x 800 像素
存储机载 & USB 驱动器
软件Countess™ 3/3 FL Cell Counter Software
尺寸9 in. (228.6 mm) W x 5.5 in. (139.7 mm) D x 9 in. (228.6 mm) W
重量(英制)8 lb.
重量(公制)3.6 kg
认证/合规RoHS
运输条件环境
质保期AB Repair Center (ABRC) Extended Warranty
频率50/60 Hz
电压100-240 VAC
Unit Size1 each
内容与储存
• Countess™ 3 FL Automated Cell Counter, 1 each
• Power cord with 4 adapters (for US/Canada/Taiwan/Japan, Europe, or UK), 1 each
• Countess™ Cell Counting Chamber Slides, 50 slides/box
• Countess™ 3 FL Disposable Slide Holder, 1 each
• Countess™ 3 FL Reusable Slide Holder, 1 each
• Countess™ 3 FL Light Cube Removal Tool, 1 each
• Countess™ 3 USB Drive, 1 each
• Countess™ 3 FL Automated Cell Counter Quick Reference Card, 1 each
• Wi-Fi Dongle (Cat. No. A26774), 1 each
• EVOS™ Light Cube, GFP 2.0 (Cat. No. AMEP4951), 1 each
• EVOS™ Light Cube, RFP 2.0 (Cat. No. AMEP4952), 1 each
• Countess™ Reusable Slide (Cat. No. A25750), 1 each
• Disposable Countess™ Cell Counting Chamber Slides (Cat. No. C10283), 10 x 50 slides
• Trypan blue (Cat. No. T10282), 20 x 1 mL vials

Store at room temperature.