TrueCut™ HiFi Cas9 Protein (1 μg/μL)
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TrueCut™ HiFi Cas9 Protein (1 μg/μL)
Invitrogen™

TrueCut™ HiFi Cas9 Protein (1 μg/μL)

Invitrogen TrueCut HiFi Cas9 Protein is a high fidelity CRISPR Cas9 protein variant engineered to demonstrate superior off-target profiles, whileRead more
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Catalog NumberQuantityConcentration
A5057525 μg1 μg/μL
A5057410 μg1 μg/μL
Catalog number A50575
Price (CNY)
982.00
Online Exclusive
Ends: 31-Dec-2025
1,262.00
Save 280.00 (22%)
Each
Add to cart
Quantity:
25 μg
Concentration:
1 μg/μL
Price (CNY)
982.00
Online Exclusive
Ends: 31-Dec-2025
1,262.00
Save 280.00 (22%)
Each
Add to cart

Invitrogen TrueCut HiFi Cas9 Protein is a high fidelity CRISPR Cas9 protein variant engineered to demonstrate superior off-target profiles, while preserving maximum on-target editing efficiency. TrueCut HiFi Cas9 Protein is ideal for applications that are sensitive to off-target events and when more precise editing is required.

Features of TrueCut HiFi Cas9 Protein include:

  • Near complete elimination of off-target events in all tested cell lines including standard, immune, primary, and stem
  • Maintains maximum on-target editing efficiency compared to TrueCut Cas9 Protein v2
  • High quality—manufactured under strict ISO 13485 quality standards
  • Transfection-ready, using either electroporation or lipid-mediated transfection reagents
  • Eliminates time-consuming cloning steps
  • Available in 2 concentrations:
    • 1 μg/μL for use in standard editing scenarios
    • 5 μg/μL for optimization of editing conditions in more difficult scenarios such as in primary or embryonic cells, microinjection, or when screening multiple gRNA sequences at a time See additional concentration
    • For custom sizes or concentrations, please inquire

TrueCut HiFi Cas9 Protein is an engineered recombinant Streptococcus pyogenes Cas9 protein, purified from E. coli, for genome editing with CRISPR technology. Cas9 protein forms a very stable ribonucleoprotein (RNP) complex with the guide RNA (gRNA) component of the CRISPR/Cas9 system. Incorporation of nuclear localization signals (NLS) aids delivery to the nucleus, increasing the rate of genomic DNA cleavage.

Options for ordering CRISPR gRNA for use with TrueCut HiFi Cas9 Protein:

  1. Order pre-designed TrueGuide Synthetic gRNAs for high-efficiency knock out in human or mouse
  2. Submit your own design for custom synthesis of CRISPR sgRNA
  3. Use our free TrueDesign CRISPR Genome Editor software for easy design and ordering of custom gRNA for precise genome editing
  4. Generate transfection-ready gRNA in as little as four hours including template assembly with our GeneArt Precision gRNA Synthesis Kit
Specifications
Product TypeHiFi Cas9
Purity≥90%
Quantity25 μg
SourceE. coli
Concentration1 μg/μL
FormLiquid
Sensitive to Heat InactivationYes
Unit SizeEach

Frequently asked questions (FAQs)

How is Gibco CTS HiFi Cas9 Protein (Cat. No. A54223, A54224) different from Invitrogen TrueCut HiFi Cas9 Protein (Cat. No. A50574, A50575)?

Both Gibco CTS HiFi Cas9 Protein (Cat. No. A54223, A54224) and Invitrogen TrueCut HiFi Cas9 Protein (Cat. No. A50574, A50575) are Streptococcus pyogenes high-fidelity CRISPR Cas9 proteins, produced by recombinant DNA technology in E. coli. Both proteins have the same sequence with added nuclear localization signal. Gibco CTS HiFi Cas9 Protein is manufactured to meet the standards for “Ancillary Materials for Cell, Gene, and Tissue-Based Products” whereas Invitrogen TrueCut HiFI Cas9 Protein is a research-grade product.

Do you offer TrueCut HiFi Cas9 Protein at different concentrations?

Yes, we offer TrueCut HiFi Cas9 Protein in the following concentrations:
1 µg/µL (Cat. Nos. A50574, A50575) for use in standard editing scenarios 5 µg/µL (Cat. Nos. A50576, A50577) for optimization of editing conditions in more difficult scenarios such as in primary or embryonic cells, microinjection, or when screening multiple gRNA sequences at a time

What is the difference between TrueCut Cas9 Protein v2 and TrueCut HiFi Cas9 Protein?

TrueCut HiFi Cas9 Protein is a modified Cas9 enzyme engineered to exhibit superior off-target profile, while maintaining maximum on-target cleavage efficiency of the wild type TrueCut Cas9 Protein v2. TrueCut HiFi Cas9 Protein is ideal for experiments that are sensitive to off-target events while still requiring a high level of editing efficiency.

What is the storage buffer composition for TrueCut Cas9 Protein v2 and TrueCut HiFi Cas9 Protein?

The storage buffer composition for TrueCut Cas9 Protein v2 and TrueCut HiFi Cas9 Protein is as follows:
15 mM Tris-HCl pH 8.0, 150 mM NaCl, 1.0 mM TCEP, 50% glycerol.

Which lipid transfection reagent do you recommend using with TrueCut Cas9 Protein v2 and TrueCut HiFi Cas9 Protein?

We recommend using Lipofectamine CRISPRMAX Transfection Reagent with TrueCut Cas9 Protein v2 or TrueCut HiFi Cas9 Protein.

View all TrueCut™ HiFi Cas9 Protein (1 μg/μL), 25 μg FAQs