TrueCut™ HiFi Cas9 蛋白 (1 μg/μL)
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TrueCut™ HiFi Cas9 蛋白 (1 μg/μL)
Invitrogen™

TrueCut™ HiFi Cas9 蛋白 (1 μg/μL)

Invitrogen TrueCut HiFi Cas9 蛋白是一种高保真 CRISPR Cas9 蛋白变体，经工程改造可展现出卓越的脱靶特征，同时可保持极高的靶向编辑效率。TrueCut HiFi了解更多信息
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货号数量最大浓度
A5057525 µg1 μg/μL
A5057410 µg1 μg/μL
货号 A50575
价格（CNY)
982.00
飞享价
Ends: 31-Dec-2025
1,262.00
共减 280.00 (22%)
Each
添加至购物车
数量:
25 µg
最大浓度:
1 μg/μL
价格（CNY)
982.00
飞享价
Ends: 31-Dec-2025
1,262.00
共减 280.00 (22%)
Each
添加至购物车

Invitrogen TrueCut HiFi Cas9 蛋白是一种高保真 CRISPR Cas9 蛋白变体，经工程改造可展现出卓越的脱靶特征，同时可保持极高的靶向编辑效率。TrueCut HiFi Cas9 蛋白非常适合用于对脱靶事件敏感的应用和需要进行更精确的编辑时。

TrueCut HiFi Cas9 蛋白的特点包括：

  • 在所有检测的细胞系（包括标准、免疫、原代以及干细胞）中几乎无脱靶事件
  • 与 TrueCut Cas9 蛋白 v2 相比，可保持极高的靶向编辑效率
  • 高质量—按照严格的 ISO 13485 质量标准生产
  • 转染即用型，使用电穿孔或脂质介导的转染试剂
  • 无需耗时的克隆步骤
  • 有 2 种浓度可供选择：
    • 1 μg/μL，用于标准编辑情况
    • 5 μg/μL，用于在更困难情况下优化编辑条件，例如原代或胚胎细胞中、显微注射时或一次筛选多个 gRNA 序列时 参见其他浓度 >
    • 对于定制大小或浓度，请咨询相关销售人员

TrueCut HiFi Cas9 蛋白是经过工程改造的重组化脓性链球菌 Cas9 蛋白，从大肠杆菌中纯化而来，可用于基于 CRISPR 技术的基因组编辑。Cas9 蛋白可与向导 RNA (gRNA) 形成一种非常稳定的核糖核蛋白 (RNP) 复合物，作为 CRISPR/Cas9 系统的一种组分。核定位信号（NLS）的引入可帮助其入核，增加基因组DNA切割的几率。

订购与 TrueCut HiFi Cas9 蛋白配合使用的 CRISPR gRNA 的可选产品：

  1. 订购用于在人或小鼠中实现高效敲除的预设计 TrueGuide 合成 gRNA
  2. 提交您自己的设计以进行 CRISPR sgRNA 定制合成
  3. 使用我们的免费 TrueDesign CRISPR 基因组编辑器软件轻松完成设计，并可订购自定义 gRNA，以实现精确的基因组编辑
  4. 只需四小时便可生成转染即用型 gRNA，包括通过我们的 GeneArt 精确 gRNA 合成试剂盒完成的模板组装。
规格
产品类型HiFi Cas9
纯度≥90%
数量25 µg
来源大肠杆菌
最大浓度1 μg/μL
形式重组蛋白
对热失活敏感是
Unit SizeEach

常见问题解答 (FAQ)

How is Gibco CTS HiFi Cas9 Protein (Cat. No. A54223, A54224) different from Invitrogen TrueCut HiFi Cas9 Protein (Cat. No. A50574, A50575)?

Both Gibco CTS HiFi Cas9 Protein (Cat. No. A54223, A54224) and Invitrogen TrueCut HiFi Cas9 Protein (Cat. No. A50574, A50575) are Streptococcus pyogenes high-fidelity CRISPR Cas9 proteins, produced by recombinant DNA technology in E. coli. Both proteins have the same sequence with added nuclear localization signal. Gibco CTS HiFi Cas9 Protein is manufactured to meet the standards for “Ancillary Materials for Cell, Gene, and Tissue-Based Products” whereas Invitrogen TrueCut HiFI Cas9 Protein is a research-grade product.

Do you offer TrueCut HiFi Cas9 Protein at different concentrations?

Yes, we offer TrueCut HiFi Cas9 Protein in the following concentrations:
1 µg/µL (Cat. Nos. A50574, A50575) for use in standard editing scenarios 5 µg/µL (Cat. Nos. A50576, A50577) for optimization of editing conditions in more difficult scenarios such as in primary or embryonic cells, microinjection, or when screening multiple gRNA sequences at a time

What is the difference between TrueCut Cas9 Protein v2 and TrueCut HiFi Cas9 Protein?

TrueCut HiFi Cas9 Protein is a modified Cas9 enzyme engineered to exhibit superior off-target profile, while maintaining maximum on-target cleavage efficiency of the wild type TrueCut Cas9 Protein v2. TrueCut HiFi Cas9 Protein is ideal for experiments that are sensitive to off-target events while still requiring a high level of editing efficiency.

What is the storage buffer composition for TrueCut Cas9 Protein v2 and TrueCut HiFi Cas9 Protein?

The storage buffer composition for TrueCut Cas9 Protein v2 and TrueCut HiFi Cas9 Protein is as follows:
15 mM Tris-HCl pH 8.0, 150 mM NaCl, 1.0 mM TCEP, 50% glycerol.

Which lipid transfection reagent do you recommend using with TrueCut Cas9 Protein v2 and TrueCut HiFi Cas9 Protein?

We recommend using Lipofectamine CRISPRMAX Transfection Reagent with TrueCut Cas9 Protein v2 or TrueCut HiFi Cas9 Protein.

View all TrueCut™ HiFi Cas9 蛋白 (1 μg/μL) FAQs