TrueCut™ HiFi Cas9 Protein (5 μg/μL), 100 μg - FAQs

查看更多产品信息 TrueCut™ HiFi Cas9 Protein (5 μg/μL) - FAQs (A50576, A50577)

5 个常见问题解答

Do you offer TrueCut HiFi Cas9 Protein at different concentrations?

Yes, we offer TrueCut HiFi Cas9 Protein in the following concentrations:
1 µg/µL (Cat. Nos. A50574, A50575) for use in standard editing scenarios 5 µg/µL (Cat. Nos. A50576, A50577) for optimization of editing conditions in more difficult scenarios such as in primary or embryonic cells, microinjection, or when screening multiple gRNA sequences at a time

What is the difference between TrueCut Cas9 Protein v2 and TrueCut HiFi Cas9 Protein?

TrueCut HiFi Cas9 Protein is a modified Cas9 enzyme engineered to exhibit superior off-target profile, while maintaining maximum on-target cleavage efficiency of the wild type TrueCut Cas9 Protein v2. TrueCut HiFi Cas9 Protein is ideal for experiments that are sensitive to off-target events while still requiring a high level of editing efficiency.

What is the storage buffer composition for TrueCut Cas9 Protein v2 and TrueCut HiFi Cas9 Protein?

The storage buffer composition for TrueCut Cas9 Protein v2 and TrueCut HiFi Cas9 Protein is as follows:
15 mM Tris-HCl pH 8.0, 150 mM NaCl, 1.0 mM TCEP, 50% glycerol.

Which lipid transfection reagent do you recommend using with TrueCut Cas9 Protein v2 and TrueCut HiFi Cas9 Protein?

We recommend using Lipofectamine CRISPRMAX Transfection Reagent with TrueCut Cas9 Protein v2 or TrueCut HiFi Cas9 Protein.

What gRNA format do you recommend using with TrueCut Cas9 Protein v2 and TrueCut HiFi Cas9 Protein?

TrueCut Cas9 Protein v2 and TrueCut HiFi Cas9 Protein are designed to work with TrueGuide synthetic gRNAs, but can also be used with in vitro transcribed gRNA from the GeneArt Precision gRNA Synthesis Kit.