Qubit™ 蛋白和蛋白宽范围 (BR) 检测试剂盒
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Qubit™ 蛋白和蛋白宽范围 (BR) 检测试剂盒
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Qubit™ 蛋白和蛋白宽范围 (BR) 检测试剂盒

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用于基于荧光的蛋白定量测定检测的 Qubit 蛋白和蛋白 BR 检测试剂盒可实现对蛋白的灵敏检测和低蛋白间差异。
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货号检测定量范围足够用于
A50668Qubit 蛋白宽范围检测试剂盒0.1 至 20 mg/mL100 次反应
Q33211Qubit 蛋白检测试剂盒12.5 µg/mL 至 5 mg/mL100 次反应
Q33212Qubit 蛋白检测试剂盒12.5 µg/mL 至 5 mg/mL500 次反应
A50669Qubit 蛋白宽范围检测试剂盒0.1 至 20 mg/mL500 次反应
货号 A50668
价格(CNY)
1,670.00
Each
添加至购物车
检测:
Qubit 蛋白宽范围检测试剂盒
定量范围:
0.1 至 20 mg/mL
足够用于:
100 次反应
价格(CNY)
1,670.00
Each
添加至购物车

使用两种独特的 Qubit 蛋白定量试剂盒快速准确地定量蛋白:Qubit 蛋白检测(定量 12.5 μg/mL 至 5 mg/mL 蛋白)和 Qubit 蛋白 BR 检测试剂盒(定量 0.1 至 20 mg/mL 蛋白)。这两种检测均可兼容多种常见污染物(还原试剂、盐和核酸)。蛋白 BR 检测试剂盒也可耐受去污剂。

Qubit 蛋白检测试剂盒
Qubit 蛋白检测试剂盒专门设计用于配合任何 Qubit 荧光计使用。使用 1-20 µL 样品时,该检测可定量测定 12.5 µg/mL 至 5 mg/mL 范围内的样品,并且蛋白间差异较低。

Qubit 蛋白检测试剂盒对蛋白具有高度选择性,并且在存在还原试剂但不存在大量去污剂的情况下具有高准确度。该检测对于常见污染物(如还原试剂(例如 DTT、β-巯基乙醇)、盐、游离核苷酸、氨基酸、溶剂或 DNA 等,但不包括去污剂)具有很好的适应性。用于其他污染物时需要轻微修改方案。

该试剂盒提供浓缩的检测试剂、稀释缓冲液和预稀释的 BSA 标准品。只需使用提供的缓冲液对试剂进行稀释,加入您的样品(1 µL 至 20 µL 的任意体积均可接受),然后即可读取浓度。

应选择哪种产品进行荧光蛋白定量测定?
•1–20 份样品:将该 Qubit 蛋白检测试剂盒与 Qubit 荧光计配合使用
• 20–2000 份样品:将 Quant-iT 蛋白检测试剂盒与微孔板读数仪配合使用

Qubit 蛋白 BR 检测试剂盒
Qubit 蛋白 BR 检测试剂盒提供了一种快速、简便、可靠的方法,使用 Qubit 4 荧光计来测定纯化蛋白、裂解物、血清、血浆或复杂蛋白混合物的蛋白浓度。该蛋白试剂盒可在较宽范围 (0.1–20 mg/mL) 内进行准确的蛋白定量测定,使大多数样品能够以纯品(未稀释)形式使用,免除了传统蛋白定量测定方法所需的推测工作和稀释步骤。该检测可兼容多种常见污染物,如去污剂、还原试剂(例如 DTT、β-巯基乙醇)、盐和核酸。

Qubit 蛋白 BR 检测试剂盒的主要特点包括:
宽动态范围—0.1-20 mg/mL,测定样品时无需稀释
两点标准曲线—免除耗时的标准品制备
自动浓度测定—即时接收蛋白浓度;无需离线计算
兼容—在存在还原试剂、去污剂和其他常见缓冲液组分时可保持准确度

Qubit 蛋白 BR 检测试剂盒配有两种校准标准品、蛋白 BR 检测缓冲液和蛋白 BR 检测试剂。只需将 150–160 µL 蛋白 BR 检测缓冲液添加至 10 或 20 µL 样品中,然后添加 30 µL 蛋白 BR 检测试剂。在室温下孵育10分钟。然后,将样品插入 Qubit 4 仪器并读取浓度。


• Qubit 蛋白检测试剂盒可与 Qubit 1.0、Qubit 2.0、Qubit 3、Qubit 4 以及 Qubit Flex 荧光计配合使用
• Qubit 蛋白 BR 检测试剂盒是一种基于荧光的检测试剂盒,仅可与 Qubit 4 荧光计配合使用
• 需要 500 µL 薄壁透明 PCR 管(货号Q32856),但其未包含在蛋白检测和 BR 检测试剂盒中

仅供科研使用。不可用于诊断程序。
规格
检测Qubit 蛋白宽范围检测试剂盒
适用于(设备)Qubit 4 荧光计
环保功能Less waste
产品线Qubit™
产品类型蛋白 BR 检测试剂盒
定量范围0.1 至 20 mg/mL
数量100 次检测
运输条件湿冰
足够用于100 次反应
检测方法荧光
Unit SizeEach

常见问题解答 (FAQ)

Is 1X RIPA buffer (50 mM Tris-HCl, 150 mM NaCl, 0.25% deoxycholic acid, 1% NP-40, 1mM EDTA, pH 7.4) compatible with the Qubit Protein Assay (Cat. No. Q33211/Q33212)?

The concentration of detergents in 1x RIPA buffer is quite high, therefore, we do not recommend that you use this kit with samples in RIPA. The Qubit Protein Assay has a tolerance for detergents present only in very low amounts. Please see the user guide for the tolerance of this assay for detergents here. The Qubit Protein Broad Range (BR) Assay Kit (A50668) has a much higher tolerance for detergents, e.g. RIPA buffer can be used undiluted. Please see this link.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Quantitation Support Center.

How can I use the Qubit Protein Broad Range Assay (Cat. No. A50668) on the Qubit 4 Fluorometer, with WiFi (Cat. No. Q33238)?

To be able to use the Qubit Protein Broad Range Assay (Cat. No. A50668) on the Qubit 4 Fluorometer (Cat. No Q33238) you would need to install the latest firmware on the fluorometer. Please find the latest Qubit 4 firmware (v2.19) on the following webpage: https://downloads.thermofisher.com/Qubit4/v2.19/Qubit4_update_v2.19.pak Save the firmware update on a USB stick and upload it to the Qubit 4 Fluorometer. Once the firmware update has been installed, you will be able to see the Protein BR assay on the Qubit 4 Fluorometer.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Quantitation Support Center.

Is the Quant-iT Protein Assay Kit (Cat. No. Q33210) a high-throughput version of Qubit Protein and Protein Broad Range Assay Kits?

Though the Quant-iT Protein Assay Kit is indicated for high-throughput application, it is completely separate from the Qubit Protein and Protein Broad Range Assay Kits, not another version of them.

Are the reagents in the Qubit Protein and Protein Broad Range Assay Kits and those from the Quant-iT Protein Assay Kit (Cat. No. Q33210) the same/interchangeable?

No, the Quant-it and Qubit reagents use different dyes and therefore are not interchangeable.

How do I get "Protein Broad Range" to appear in the Qubit 4 Fluorometer “Protein” menu required to read the standards and samples for the Qubit Protein BR Assay Kit (Cat. Nos. A50668, Q33211, Q33212, A50669)?

To access “Protein Broad Range” in the “Protein” menu of your Qubit 4 instrument, as instructed in the Qubit Protein BR Assay Kit (Cat. Nos. A50668, Q33211, Q33212, A50669) instructions, the instrument will require the latest firmware update (v2.19 or v1.8.0 for the Qubit Flex). You can check which version you currently have on your device by clicking on "Settings" from the Home screen, and then on "About Instrument".

To update the software:

Download the latest firmware update from the Qubit Fluorometers Technical Resources page.
Follow the instructions for updating your Qubit instrument in the firmware Release notes or in the Qubit 4 Fluorometer User Guide (page 53).

After the update has been completed, the option "Protein Broad Range" should become available.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Quantitation Support Center.