AccelerOme™ TMT11plex™ MS 样品制备试剂盒,30–100 μg
AccelerOme™ TMT11plex™ MS 样品制备试剂盒,30–100 μg
Thermo Scientific™

AccelerOme™ TMT11plex™ MS 样品制备试剂盒,30–100 μg

Thermo Scientific Accelerome TMT11plex MS 样品制备试剂盒(30–100 µg)提供了可高效稳健地进行蛋白质组学质谱 (MS)了解更多信息
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货号数量足够用于
A509463 x 11 次反应10–30 μg samples
A509471 x 11 次反应30–100 μg samples
A509483 x 11 次反应30–100 μg samples
货号 A50946
价格(CNY)
22,994.00
Each
添加至购物车
数量:
3 x 11 次反应
足够用于:
10–30 μg samples
价格(CNY)
22,994.00
Each
添加至购物车
Thermo Scientific Accelerome TMT11plex MS 样品制备试剂盒(30–100 µg)提供了可高效稳健地进行蛋白质组学质谱 (MS) 样品制备的完整解决方案(包括 TMT 肽标记),以与 AccelerOme 自动样品制备系统配合使用。

TMT11plex MS 样品制备试剂盒 (30–100 µg) 的特点包括:
经过优化—EasyPep 试剂盒化学试剂与 TMT11plex 标记试剂结合,专门设计与 AccelerOme 自动样品制备系统配合使用
便利—整套即用型蛋白质组学样品制备、纯化和 TMT 标记试剂,易于加载到仪器中
稳健—试剂与 AccelerOme 生态系统相结合,可提供可重现的高质量 TMT 标记肽,标记效率大于 95%
灵活—在系统上使用细胞、血浆、蛋白和组织样品(上样量 30–100 µg)对样品制备和 TMT 标记试剂进行了验证
多通路—可同时对多达 11 份样品进行 MS 分析

AccelerOme TMT11plex MS 样品制备试剂盒集成了预配制缓冲液、MS 级胰蛋白酶/Lys-C 酶混合物和带有 TMT11plex 标记试剂的肽纯化柱,用于 TMT 标记肽的可重现样品制备。已对 TMT11plex 标记试剂进行了优化,以与 30–100 µg 样品配合使用,达到大于 95% 的标记效率。这些单次使用组分在贴标签的托盘中提供,使用方便,可直接放置在 AccelerOme 系统上。此外,该试剂盒还提供肽保留时间校准标准品(单独发运),可在 LC-MS 分析前加入样品中评估系统性能。该试剂盒提供了完整的 TMT11plex 标记样品自动样品制备试剂解决方案,可生成高质量的 MS 即用肽。

AccelerOme 试剂产品组合是 AccelerOme 自动化系统解决方案的组成部分,可进行稳健的蛋白质组学质谱样品制备。仪器硬件基于经验证的液体处理形式构建,可实现可重现的样品处理和优化的在线 μSPE 样品纯化,获得出色的肽回收率。在样品纯化前,已进行 TMT 标记复溶、样品标记和混合。通过精确的紫外分光光度分析进行肽纯化后定量,以在 LC-MS 分析之前测定肽浓度。整套系统(试剂盒 + 试剂 + 软件)旨在提供高质量的肽,同时通过易于使用的自动化系统提高生产率。与行业前列的高分辨率 Orbitrap 仪器和软件结合使用时,TMT11plex 试剂可为定量蛋白表达分析提供综合全面的解决方案。

这些产品受限于限制性使用标记许可。

规格
最终产品类型TMT-labeled peptide sample preparation
套装内容Sample prep module, TMT module
数量3 x 11 次反应
足够用于10–30 μg samples
工作流程步骤Automated mass spec sample prep
检测方法紫外吸收
产品规格试剂盒
产品线Thermo Scientific™
产品类型试剂盒
原始材料蛋白样本
Unit SizeEach

常见问题解答 (FAQ)

Can I use the AccelerOme TMT11plex MS Sample Preparation Kits and the AccelerOme TMTpro 16plex MS Sample Preparation Kits for samples with protein amounts lower than 10 µg?

Unfortunately, the AccelerOme TMT11plex MS Sample Preparation Kits and the AccelerOme TMTpro 16plex MS Sample Preparation Kits have not been validated for use with sample lysates containing lower than 10 µg of initial protein.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

With the AccelerOme TMT11plex MS Sample Preparation Kits and the AccelerOme TMTpro 16plex MS Sample Preparation Kits, can I use any other lysis buffer instead of the one in the kit?

We do not recommend using a different lysis buffer other than the one included in the kit. On the one hand, variation in the lysis buffer might affect the subsequent sample prep steps. On the other hand, volume of the different buffers included in the kit has been optimized for the correct performance of the Accelerome platform.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

When using the AccelerOme TMT11plex MS Sample Preparation Kits and the AccelerOme TMTpro 16plex MS Sample Preparation Kits with the AccelerOme platform, can I stop the process at any time?

We do not recommend stopping the automatized process as the AccelerOme platform is programmed to perform the complete sample preparation in a single run while keeping sample stability and reproducibility as its highest.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

When using the AccelerOme TMT11plex MS Sample Preparation Kits and the AccelerOme TMTpro 16plex MS Sample Preparation Kits, should I add a protease inhibitor cocktail to the lysis buffer? How about phosphatase inhibitors?

We do not recommend using protease inhibitor cocktails containing EDTA with the AccelerOme TMT11plex MS Sample Preparation Kits and the AccelerOme TMTpro 16plex MS Sample Preparation Kits as they will inhibit both Universal nuclease and protease Trypsin/Lys-C activities. Following cell lysis, reduction and alkylation, the Trypsin/Lys-C proteases mix is added for complete digestion. Therefore, if protease inhibitors containing EDTA are added, there will be incomplete proteolysis. On the other hand, we do recommend using a phosphatase inhibitor cocktail (e.g., Halt Phosphatase Inhibitor Cocktail) if a subsequent phosphopeptide enrichment analysis will be performed.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

Can I run the samples obtained from the AccelerOme TMT11plex MS Sample Preparation Kits and the AccelerOme TMTpro 16plex MS Sample Preparation Kits in any other mass spectrometer besides Orbitrap Mass Spectrometers?

We recommend using Orbitrap Mass Spectrometers as the analysis of TMT reagents requires a system that is able to perform high-resolution MS/MS fragmentation. Orbitrap Mass Spectrometers include a specific TMT MS2 workflow which enables unique acquisition management resulting in confident results without compromising coverage.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.