Multiskan SkyHigh Microplate Spectrophotometer - FAQs

查看更多产品信息 Multiskan SkyHigh Microplate Spectrophotometer - FAQs (A51119700DPC, A51119600DPC, A51119600C, A51119700C, A51119500C)

3 个常见问题解答

Can you please let me know which plates with and without lid are compatible with the Multiskan SkyHigh Microplate Spectrophotometer?

The Multiskan SkyHigh Microplate Spectrophotometer supports clear bottom 6- to 48-, 96- and 384-well microplates with and without lids, where the maximum height of the microplate or a combination of the microplate and lid is 19.5 mm. Please refer to the Plate Selection Table for Plate Readers brochure (https://assets.thermofisher.com/TFS-Assets/BID/Flyers/nunc-plate-selection-table-plate-readers-flyer.pdf) for specific plates to use.

You can also see the specific plate types in SkanIt Software v7.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Which is the best DNA or RNA quantification instrument?

All of the DNA instruments offered by Thermo Fisher Scientific are of excellent quality. To find the right instrument to quantify DNA or RNA in your lab, explore the detailed comparison of UV-Vis spectrophotometers and fluorometers. Things like sensitivity, throughput and budget may be initial considerations when selecting a DNA quantification instrument. You may also consider whether you need target specificity, sample purity information, RNA quality information, or broad dynamic range.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Quantification Support Center.

What is the difference between UV and fluorescence DNA quantification? Which DNA quantification technology should I choose?

UV and fluorescence technologies work differently to quantify DNA. UV quantification relies on the intrinsic absorptivity of DNA and RNA molecules, while fluorescence quantification uses dyes that specifically bind to your molecule of choice. With UV technology, quantification isn't as sensitive, but it has a broader dynamic range and also gives data about sample purity‒plus it is faster because there is no reagent prep. With fluorescence technology you get higher sensitivity and molecule-specific data, but it has lower dynamic range and reagent prep is required. The technology you need will depend on what features are important for your lab and experimentation.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Quantification Support Center.