SureQuant™ Protein A/G IP-MS Sample Preparation Kit, 20 Reactions - FAQs

查看更多产品信息 SureQuant™ IP-MS Sample Preparation Kits - FAQs (A51743, A51744)

3 个常见问题解答

Why is the processing time for MS analysis less with SureQuant IP-MS Sample Preparation kits compared to traditional IP methods?

SureQuant Protein IP-MS Sample Preparation kits use a trypsin enzyme elution method to dissociate the bound immune complexes from the magnetic beads, which can then be digested directly in-solution and followed up by MS analysis on the same day; no SDS-PAGE purification is required. The entire process take 4 hours compared to traditional IP methods that usually take around 21 hrs.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

What is the difference between SureQuant IP-MS Sample Preparation kits and Pierce MS-Compatible Magnetic IP kits?

SureQuant IP-MS Sample Preparation kits include all reagents necessary for sample lysis, immunoenrichment, elution, digestion, and MS analysis. The entire process is completed in 4 hr for same-day LC-MS/MS analysis as compared to traditional IP methods that usually take around 21 hr. Pierce MS-Compatible Magnetic IP kits provide reagents necessary for the lysis step and the elution step, but not for the digestion step prior to MS analysis.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

Can I use SureQuant Protein A/G and SureQuant Streptavidin IP-MS Sample Preparation kits with any sample?

SureQuant IP-MS sample preparation kits have been designed for use with SureQuant quantitation modules for upstream sample preparation for SRM/PRM analysis, but they can be used with any samples where the target protein needs to be enriched by IP or IP-CoIP, prior to MS analysis.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.