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查看更多产品信息 SureQuant™ Phosphopeptide Suitability Standards - FAQs (A51745)
3 个常见问题解答
The SureQuant Phosphopeptide Suitability Standard is designed for calibration of the MS Instrument whereas the SureQuant Multipathway Phosphopeptide Standard is designed for internal quantitation and analysis of specific peptides. The 20 phosphopeptides present in the SureQuant Phosphopeptide Suitability Standard are included within the SureQuant Multipathway Phosphopeptide Standard.
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The SureQuant Multipathway Phosphopeptide Standard can be used with any MS Instrument that is capable of Tandem MS (MS/MS, MS2). The SureQuant Multipathway Phosphopeptide Standard can be used for internal quantitation and analysis of specific peptides
Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.
The SureQuant Multipathway Phosphopeptide Standard is an optimized mixture of 131 isotopically labeled HeavyPeptide AQUA Basic phosphopeptides from 89 key signaling proteins. The specific phosphopeptides have been chosen to cover biologically relevant phosphorylation sites from seven different signaling pathways including EGFR/HER, RAS-MAPK, PI3K/AKT/mTOR, AMPK, death/apoptosis, and stress (p38/SAPK/JNK) signaling, in order to simultaneously monitor and quantitate phosphorylation signaling using LC-MS/MS analysis.
The SureQuant Multipathway Phosphopeptide Standard is provided at a final concentration of 100 fmol/µL for use as a spike-in standard for MS proteomic samples. Differences in peak height of each peptide in the mix in LC-MS/MS analysis are due to differences in charge state, solubility, matrix effect, and hydrophilicity/hydrophobicity.
Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.