TMTpro 18-plex、TMTpro-134C 和 TMTpro-135N 标记试剂
TMTpro 18-plex、TMTpro-134C 和 TMTpro-135N 标记试剂
Thermo Scientific™

TMTpro 18-plex、TMTpro-134C 和 TMTpro-135N 标记试剂

TMTpro 18plex 标记试剂实现对多达18份样品的多通路检测。添加 TMTpro 134C 和 TMTpro 135N 标记试剂可将样品多通路从16通路增加至18通路,甚至提高了通过串联质谱 (MS) 法进行蛋白鉴别和定量测定的通量。
Have Questions?
更改视图buttonViewtableView
货号数量标签或染料
A520455 mgTMTpro 18-plex
A520465 mgTMTpro-134C and TMTpro-135N
A520476 x 0.5 mgTMTpro 18-plex
A520486 x 0.5 mgTMTpro-134C and TMTpro-135N
A583354 x 50 μg (per tag)Tandem Mass Tag™ (TMT) 标记
货号 A52045
价格(CNY)
154,694.00
Each
添加至购物车
数量:
5 mg
标签或染料:
TMTpro 18-plex
价格(CNY)
154,694.00
Each
添加至购物车

TMTpro 标记试剂是新一代串联质谱标签,设计用于提高样品多通路水平,同时不影响蛋白鉴定和定量。Thermo Scientific TMTpro 18plex 标记试剂套件实现对多达18份样品的多通路检测,甚至提高了通过串联质谱 (MS) 法进行蛋白鉴别和定量分析的通量。

TMTpro 和 TMT 标签的结构在同量异位和胺反应性方面相似,但 TMTpro 标签具有更长的间隔区和异丁基脯氨酸质量报告基因。在 MS/MS 片段化之后,每个 TMTpro 18plex 标签在高分辨率 MS/MS 质谱的低质量区域生成一个独特报告基因质量(例如 TMTpro 126-135 Da),用于蛋白表达水平的相对定量测定。

TMT pro134C 和 TMTpro135N 标记试剂的组成和结构与其他 TMTpro 16plex 标签相同,但不是同量异位。添加 TMTpro 134C 和 135N 标签创建了第17和第18通道,可使用高分辨率 Orbitrap 质谱仪进行相对定量测定。这些标签单独提供(货号:A52046、A52048)或作为 TMTpro 18plex 标记试剂套件的一部分提供。

TMTpro 18plex 标记试剂套件的特点包括:
多通路—对源自细胞、组织或生物体液的多达18份样品进行并行 MS 分析
稳健—多通路能力提高,可减少样品中定量值的缺失,并提高重复试验之间的置信度
高效—胺反应性、NHS 酯活化试剂可确保所有肽的标记率超过 95%,无论蛋白质序列或蛋白水解酶特异性如何
经优化可与高分辨率 Thermo Scientific MS/MS Orbitrap 平台配合使用,并且 Proteome Discoverer 2.3 完全支持分析
便利的规格—以即用型、一次性使用形式提供,可确保良好稳定性和性能

为对 TMTpro 标记肽进行高分辨率分析,推荐用于 Nanospray Flex 离子源的 LC 色谱柱是 Acclaim PepMap 100 C18 LC 柱(货号:164942 或 164939)。对于 EASY-Spray 离子源,推荐 LC 色谱柱为 EASY-Spray C18 LC 色谱柱(货号 ES902 或 ES903)。结合行业领先的高分辨率 Orbitrap 仪器和软件使用时,TMTpro 试剂可为定量蛋白表达分析提供综合完整的解决方案。

仅供科研使用。不可用于诊断程序。
规格
标签或染料TMTpro 18-plex
数量5 mg
工作流程步骤肽标记与定量
检测方法质谱分析
产品类型同量异位标记试剂套件
原始材料
Unit SizeEach
内容与储存
保存于 –20°C 。

常见问题解答 (FAQ)

Can I combine TMTpro tags with TMT tags?

We do not recommend mixing TMTpro and TMT tags, as they differ in chemical structure.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

Can I purchase the TMTpro-134C and TMTpro-135N Label Reagents separately from the TMTpro 18-plex Label Reagent Set?

Yes, Cat. No. A52046 provides TMTpro-134C and TMTpro-135N tags as 1 vial each of 5 mg and Cat. No. A52048 provides TMTpro-134C and TMTpro-135N tags as 6 vials each of 0.5 mg.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

What are the components of the TMTpro 18-plex Label Reagent Sets?

The TMTpro 18-plex Label Reagent Sets are the combination of corresponding TMTpro 16 plex Label Reagent Sets and TMTpro-134C & TMTpro-135N Label Reagents. Cat. No. A52045 is a combination of Cat. No. A52046 and Cat. No. A44520 where all TMTpro tags are provided as 1 vial each of 5 mg. Cat. No. A52047 is a combination of Cat. No. A52048 and Cat. No. A44522 where all TMTpro tags are provided as 6 vials each of 0.5 mg.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

At which step should I label my mass spec samples with TMT reagents?

TMT labeling can be performed after reduction, alkylation, digestion, or prior to cleanup, if the sample is buffered at appropriate pH (8-8.5) in a suitable buffer without primary amines (e.g., Tris, glycine). TMT labeling can also be performed after peptide clean-up. Labeling of peptides after clean-up enables measuring and normalizing of the peptide samples for equal mixing.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

Do I have to apply correction factors for analyzing data for TMT/TMTpro-labeled samples?

Applying correction factors is required for more accurate relative protein quantitation as the isotopic impurity is variable among the tags, ranging from 5-10%.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.