JournalMethods in molecular biology (Clifton, N.J.)
PubMed ID39052222
Archived tumor specimens are routinely preserved by formalin fixation and paraffin embedding. Despite the conventional wisdom that proteomics might be ineffective due to the cross-linking and pre-analytical variables, these samples have utility for both discovery and targeted proteomics. Building on this capability, proteomics approaches can be used to maximize our ... More
TMTpro-18plex: The Expanded and Complete Set of TMTpro Reagents for Sample Multiplexing.
AuthorsLi J,Cai Z,Bomgarden RD,Pike I,Kuhn K,Rogers JC,Roberts TM,Gygi SP,Paulo JA
JournalJournal of proteome research
PubMed ID33900084
The development of the TMTpro-16plex series expanded the breadth of commercial isobaric tagging reagents by nearly 50% over classic TMT-11plex. In addition to the described 16plex reagents, the proline-based TMTpro molecule can accommodate two additional combinations of heavy carbon and nitrogen isotopes. Here, we introduce the final two labeling reagents, ... More