Efficient-Pro Feed 2 is a next-generation Chinese hamster ovary (CHO) feed used in the production of monoclonal antibodies (mAbs), offeringRead more
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Catalog Number
Quantity
A5221401
1000 mL
Catalog number A5221401
Price (CNY)
7,885.00
Each
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Quantity:
1000 mL
Price (CNY)
7,885.00
Each
Add to cart
Efficient-Pro Feed 2 is a next-generation Chinese hamster ovary (CHO) feed used in the production of monoclonal antibodies (mAbs), offering significant performance and ease-of-use improvements over earlier Gibco and competitor products. Designed to work optimally with Efficient-Pro Medium, Efficient-Pro Feed 2 has been formulated specifically for use in CHO-S and DG-44 cell lines. The Efficient-Pro system increases titer and specific productivity, improves protein quality and metabolite profiles, and is easier to use with less solubility issues than other CHO media and feed offerings.
The Efficient Pro system will help you: • Sustain cell viability for longer bioreactor runs and maximized titer • Improve downstream outputs such as protein glycosylation, charge-variants, and aggregation • Speed up process development and streamline transfer to manufacturing scale • Increase flexibility with a range of format and packaging configurations • Decrease cell shock with simplified adaptation protocols
Efficient-Pro Feed 2 has been formulated as a chemically defined, animal-origin-free cell culture feed used in the bioproduction of mAbs and other therapeutics in traditional batch and fed-batch bioprocessing workflows, particularly in combination with CHO cell line variants.
Efficient-Pro Feed 2 is a balanced formulation designed to meet the metabolic needs of mAb production and does not contain growth factors or insulin to ensure batch-to-batch consistency and production efficiency. Demonstrated to improve titers in a DG-44 cell line up to 146% when paired with Efficient-Pro Medium compared to an industry-leader’s media and feed system, the Efficient-Pro system helps accelerate your time-to-market and achieve success.
For Research Use Only. Not for use in diagnostic procedures.
Can I use Efficient-Pro Feed 2 with other cell lines besides CHO-S and DG-44 cell line variants?
Efficient-Pro Feed 2 has been designed specifically for CHO-S and DG-44 cells; however, it has been demonstrated to improve performance in other CHO variants and even other parental cell lines.
I currently supplement my CHO cells with insulin and other growth factors. Should I continue the use of these other additives when using Efficient-Pro Feed 2?
We recommend continuing current supplementation protocols including insulin and other growth factors when using Efficient-Pro Feed 2.
How often and at what concentration should I feed my CHO cells with Efficient-Pro Feed 2?
The comprehensive feed protocol for Efficient-Pro Feed 2 is included in the user guide (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0025906_EfficientProFeed2_UG.pdf). We recommend first designing a small-scale experiment using an Ambr15 Microbioreactor or other small vessel with concentrations between 1.5% to 3.0% of Efficient-Pro Feed 2 in 0.5% increments beginning on day 3, with daily feeds through harvest. Once the specific feeding strategy is tailored to your CHO cell system for optimized results, cultures can be scaled up to larger vessels and bioreactors.
When should I consider using Efficient-Pro Feed 2?
Efficient-Pro Feed 2 is a supplement that has been formulated to work specifically with CHO-S and DG-44 cells for production of proteins. Efficient-Pro Feed 2 has been designed to work optimally when paired with Efficient-Pro Medium (https://www.thermofisher.com/order/catalog/product/A5322201). Efficient-Pro Feed 2 has been demonstrated to improve yield and other end-points when used as described in the user guide (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0025906_EfficientProFeed2_UG.pdf).