High-Select™ Fe-NTA Magnetic Phosphopeptide Enrichment Kit - FAQs

查看更多产品信息 High Select™ Phosphopeptide Enrichment Kits & Reagents - FAQs (A52283)

4 个常见问题解答

Can I purchase High-Select Fe-NTA Magnetic beads from the High-Select Fe-NTA Magnetic Phosphopeptide Enrichment Kit as a standalone product?

Yes, the standalone beads can be purchased as High-Select Fe-NTA Magnetic Agarose, Cat. No. A52284.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

What type of samples can I use with the High-Select Fe-NTA Phosphopeptide Enrichment Kit and High-Select Fe-NTA Magnetic Phosphopeptide Enrichment Kit?

The High-Select Fe-NTA Phosphopeptide Enrichment Kit and High-Select Fe-NTA Magnetic Phosphopeptide Enrichment Kit are compatible with desalted and detergent-free protein digests or lyophilized peptide fractions.

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What is the composition of the Binding/Wash Buffer in the High-Select Fe-NTA Phosphopeptide Enrichment Kit and High-Select Fe-NTA Magnetic Phosphopeptide Enrichment Kit?

Here is the composition of the Binding/Wash Buffer in the High-Select Fe-NTA Phosphopeptide Enrichment Kit and High-Select Fe-NTA Magnetic Phosphopeptide Enrichment Kit: 0.1 % trifluoroacetic acid (TFA), 80% acetonitrile (ACN).

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After phosphopeptide enrichment using the High-Select Fe-NTA Phosphopeptide Enrichment Kit or High-Select Fe-NTA Magnetic Phosphopeptide Enrichment Kit, I see a yellow/slight brownish color material in my eluate or after drying my samples. Is this normal?

The yellow/slight brownish color is indicative of iron which has been released from the column during elution along with the phosphopeptides. After acidification of the elution buffer or resuspension of dried peptides in 0.1% formic acid, the material can be removed by centrifugation of the sample at 16,000 x g for 30 sec and transferring the supernatant into a new container for LC/MS injection or peptide quantification. Alternatively, the material can be removed using Pierce Peptide Desalting Spin Columns, Pierce C18 Spin Tips or an in-line C18 trap column.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.