In the User Guide for TaqPath BactoPure Microbial Detection Master Mix (Cat. Nos. A52699, A52700, A53701, A52702), there are pre-read and post-read steps included for setting up and running the real-time PCR plate. Are these steps required?

Question

In the User Guide for TaqPath BactoPure Microbial Detection Master Mix (Cat. Nos. A52699, A52700, A53701, A52702), there are  pre-read and post-read steps included for setting up and running the real-time PCR plate. Are these steps required?

Accepted Answer

The pre- and post-run read steps for setting up and running the real-time PCR plates using the protocol in the User Guide for TaqPath BactoPure Microbial Detection Master Mix (Cat. Nos. A52699, A52700, A53701, A52702) are not required. However, the pre-read and post-read steps listed in the table for setting up and running the plate (pg. 12) can be used to normalize data collected. TaqPath BactoPure Microbial Detection Master Mix is compatible with most TaqMan qPCR protocols.

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TaqPath™ BactoPure™ Microbial Detection Master Mix, 1 x 1 mL - FAQs

In the User Guide for TaqPath BactoPure Microbial Detection Master Mix (Cat. Nos. A52699, A52700, A53701, A52702), there are pre-read and post-read steps included for setting up and running the real-time PCR plate. Are these steps required?