MabCaptureC™ High Capacity Protein A Resin, 100 mL - FAQs

View additional product information for MabCaptureC™ High Capacity Protein A Resin - FAQs (A40004564, A53031, A40004566, A53033, A53034)

11 product FAQs found

Can I automate the workflow using MabCaptureC High Capacity Protein A Resin?

Yes. FPLC (Fast protein liquid chromatography) use is outlined in the instruction manual, along with compatible buffer formulations, proper flow rates, and clean-in-place protocols.

Using MabCaptureC High Capacity Protein A Resin, what downstream assays are compatible with the eluted samples?

Purified samples eluted from MabCaptureC High Capacity Protein A Resin are compatible with protein assays (Bradford, BCA, Rapid Gold, A280, etc.) and can be used for industry standard testing applications (western blotting, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry, etc.).

What is the maximum operational flow velocity that can be used with MabCaptureC High Capacity Protein A Resin?

The maximum operational flow velocity with MabCaptureC High Capacity Protein A Resin is 300 cm/hr or less (150 cm/hr recommended).

What is the pH stability of MabCaptureC High Capacity Protein A Resin?

MabCaptureC High Capacity Protein A Resin is stable at pH 2-14 (short-term storage) and pH 3-14 (long-term storage).

After regeneration of MabCaptureC High Capacity Protein A Resin, is there a specific buffer in which the beads should be stored?

If the beads are not going to be re-used immediately, we recommend using 20% ethanol for long-term storage.

How do I regenerate MabCaptureC High Capacity Protein A Resin?

Using the Clean-In-Place (CIP) protocol provided in the instruction manual, we recommend cleaning the MabCaptureC High Capacity Protein A Resin with 10 CV (column volumes) of 0.2 M NaOH after every run. The resin has limited stability at 0.5 M NaOH, but this can be used in an optimized cleaning strategy after every 10th or 20th cycle.

How many uses/regenerations are possible with MabCaptureC High Capacity Protein A Resin?

MabCaptureC High Capacity Protein A Resin can be regenerated and reused for over 100 times without affecting protein yield or purity (depending on the sample type).

What is the dynamic binding capacity of MabCaptureC High Capacity Protein A Resin?

The dynamic binding capacity of MabCaptureC High Capacity Protein A Resin is 60 mg hIgG/mL at 5 min residence time, going up to 80 mg hIgG/mL at 10 min residence time.

What is the particle size of the MabCaptureC High Capacity Protein A Resin?

The Protein A ligand, which is produced in yeast, is immobilized on a rigid and uniformly sized agarose bead with an average particle size of 75 µm.

What is the resin matrix in MabCaptureC High Capacity Protein A Resin?

MabCaptureC High Capacity Protein A Resin is composed of highly cross-linked agarose beads (6%).

Is MabCaptureC High Capacity Protein A Resin suited for bioprocessing?

Yes. The Alkaline-stable Protein A resin is compatible with many different formats for bioprocessing (serum, ascites, CHO cells, Expi293 cells, etc.).