Pierce™ High Capacity Protein A MagBeads, alkali stable, 4 x 25 mL - FAQs

查看更多产品信息 Pierce™ High Capacity Protein A MagBeads, alkali stable - FAQs (A53037, A53036, A53038, A53035)

5 个常见问题解答

Can I automate the workflow using Pierce High Capacity Protein A MagBeads, alkali stable?

Yes. A KingFisher Apex protocol for 96-deep well plates is provided on pages 3 and 4 in the instruction manual (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0026311_Pierce_HiCap_proteinA_MagBeads_UG.pdf).

Using Pierce High Capacity Protein A MagBeads, alkali stable, what downstream assays are compatible with the eluted samples?

Purified samples eluted from Pierce High Capacity Protein A MagBeads, alkali stable are compatible with protein assays (Bradford, BCA, Rapid Gold, A280, etc.) and can be used for industry standard testing applications (western blotting, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry, etc.).

After regeneration of Pierce High Capacity Protein A MagBeads, alkali stable, is there a specific buffer in which the beads should be stored?

If the beads are not going to be re-used immediately, we recommend using 20% ethanol for long-term storage.

How do I regenerate Pierce High Capacity Protein A MagBeads, alkali stable?

Using the Clean-In-Place (CIP) protocol provided in the instruction manual, we recommend cleaning the Pierce High Capacity Protein A MagBeads, alkali stable with 20 bed volumes of 0.1 M NaOH after every run.

How many uses/regenerations are possible with Pierce High Capacity Protein A MagBeads, alkali stable?

Pierce High Capacity Protein A MagBeads, alkali stable can be reused at least 10 times with <5% loss in binding capacity.