Efficient-Pro™ 培养基
Efficient-Pro™ 培养基
Gibco™

Efficient-Pro™ 培养基

Efficient-Pro 培养基是用于生产单克隆抗体 (mAb) 的下一代中国仓鼠卵巢 (CHO) 培养基,与之前的 Gibco 和竞争产品相比,性能和易用性显著提高。Efficient-Pro AGT了解更多信息
Have Questions?
更改视图buttonViewtableView
货号数量
A53222011000 mL
A532220210 L
A532220320 L
货号 A5322201
价格(CNY)
2,772.00
Each
添加至购物车
数量:
1000 mL
价格(CNY)
2,772.00
Each
添加至购物车
Efficient-Pro 培养基是用于生产单克隆抗体 (mAb) 的下一代中国仓鼠卵巢 (CHO) 培养基,与之前的 Gibco 和竞争产品相比,性能和易用性显著提高。Efficient-Pro AGT 系统设计为可与 Efficient-Pro AGT 补料 1 和 Efficient-Pro AGT 补料 2 充分配合使用,分别用于 CHO-K1 和 DG-44/CHO-S 细胞系,Efficient-Pro 系统可提高滴度和单位产量,改善蛋白质量和代谢物特征,与其他 CHO 培养基和补料产品相比,更易于使用,溶解度问题更少。

Efficient Pro AGT 系统将有助于:
•维持细胞活力以延长生物反应器运行时间,并尽可能提高滴度
• 改善下游输出,如蛋白糖基化、电荷变体和聚集
• 加快工艺开发并简化向生产规模的转移
• 通过一系列规格和包装配置提高灵活性
• 通过简化的适应方案降低细胞休克

Efficient-Pro 培养基已配制为化学成分明确的非动物源性细胞培养基,适用于传统批次和分批补料生物工艺工作流程中(特别是与 CHO 细胞系变体结合时)mAb 和其他治疗药物的生物生产。

Efficient-Pro 培养基是一种均衡配方,其设计符合 mAb 生产的代谢需求,且不含生长因子或胰岛素,确保批间一致性和生产效率。与处于行业前列的培养基和补料系统相比,Efficient-Pro AGT 系统与 Efficient-Pro AGT 补料 1 配合使用时,可提高 CHO-K1 细胞系中的生产率,有助于缩短上市时间并取得成功。

仅供研究使用。不可用于诊断程序。
规格
细胞系CHO-DG44、CHO-S、CHO-K1、GS-CHO
环保功能绿色可持续包装
数量1000 mL
运输条件室温
分类非动物源性, 化学成分明确, 无蛋白, 无血清
培养类型哺乳动物细胞培养
形式液体
Unit SizeEach

常见问题解答 (FAQ)

What is the shelf-life of Efficient-Pro Medium?

The shelf-life of Efficient-Pro Medium is 12 months from the date of manufacture. The expiration date is provided on the product label.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

If my cell line is sensitive to ammonia, can I still use Efficient-Pro Medium?

Yes, however, we recommend reducing the L-glutamine concentration if a decrease in cell viability is observed. The recommended glutamine concentration is 2-8 mM. Another way to reduced ammonia production is to use GlutaMAX Supplement instead of L-glutamine. Addition of Pluronic F-68 is not required.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Does Efficient-Pro Medium require supplementation for use?

When using a CHO cell line with a selection system other than the glutamine synthetase (GS) system, we recommend adding 2-8 mM L-glutamine or GlutaMAX Supplement to Efficient-Pro Medium. Additionally, we recommend making sure that glucose levels do not fall below 2 g/L in culture by supplementing with glucose as needed. Also, if clumping becomes an issue, then add anti-clumping agent (ACA) to your cultures at 1% v/v.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the concentration of glucose in Efficient-Pro Medium?

Efficient-Pro Medium contains 4.7 g/L of glucose.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can CHO cells that are created or frozen in other production media be thawed into Efficient-Pro Medium or adapted for growth and production in Efficient-Pro Medium?

Cells that are grown in or adapted to, and frozen in other cell culture media readily adapt to Efficient-Pro Medium. However, if your cells are experiencing a significant lag or delay in growth, please directly adapt the cells into Efficient-Pro Medium and passage them for a minimum of 3 passages, until a consistent doubling time is observed.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.