MagMAX™ Viral/Pathogen Nucleic Acid Isolation Kit, for HIV-1 dried blood spots (DBS) - FAQs

View additional product information for MagMAX™ Viral/Pathogen Nucleic Acid Isolation Kit, for HIV-1 dried blood spots (DBS) - FAQs (A53770)

9 product FAQs found

Is the MagMAX Dried Blood Spots Lysis Solution from the MagMAX Viral/Pathogen Nucleic Acid Isolation Kit, for HIV-1 dried blood spots (DBS) the same as the Binding Solution in the MagMAX Viral/Pathogen Nucleic Acid Isolation Kit (Cat. No. A42352, A48310)?

The MagMAX Dried Blood Spots Lysis Solution is different from the Binding Solution in the MagMAX Viral/Pathogen Nucleic Acid Isolation Kit (Cat. No. A42352, A48310).

What is the total extraction workflow time with the MagMAX Viral/Pathogen Nucleic Acid Isolation Kit, for HIV-1 dried blood spots (DBS) (Cat. No. A53770)?

The approximate extraction workflow time with the KingFisher Flex instrument is <90 min for 24 DBS samples and <60 min for 24 plasma samples. With the KingFisher Duo instrument, the approximate extraction workflow time is <75 min for 12 DBS samples and <45 min for 12 plasma samples.

What is the maximum plasma input volume for the MagMAX Viral/Pathogen Nucleic Acid Isolation Kit, for HIV-1 dried blood spots (DBS)?

The MagMAX Viral/Pathogen Nucleic Acid Isolation Kit, for HIV-1 dried blood spots (DBS) was validated using 200 µL of plasma sample. However, the standard protocol mentioned in the product manual uses reagent volumes that can work with 200-400 µL of sample.

Why is the recovered volume of Dried Blood Spots (DBS) lysate sample variable (~350-450 microL) for the MagMAX Viral/Pathogen Nucleic Acid Isolation Kit, for HIV-1 dried blood spots (DBS)?

The DBS lysate volume is variable because the filter paper material absorbs anywhere from 150-250 µL of the starting 600 µL of Dried Blood Spots Lysis Solution during incubation. If two 13 mm diameter cutouts are used instead of one, the DBS lysate volume recovered may be even less.

Which type of tubes do you recommend using for DBS lysis with the MagMAX Viral/Pathogen Nucleic Acid Isolation Kit, for HIV-1 dried blood spots (DBS) (Cat. No. A53770)?

The MagMAX Viral/Pathogen Nucleic Acid Isolation Kit, for HIV-1 dried blood spots (DBS) (Cat. No. A53770) was validated using 1.5 mL non-stick tubes per 13 mm diameter DBS cutout. However, the 1.5 mL tubes have a narrow opening which requires folding the 13 mm DBS cutout into a “taco” shape prior to inserting into the tube. Other tubes (eg., Sarstedt 8 mL) have been used during development and work well, but have more dead space which may result in more sample evaporation.

How is the MagMAX Viral/Pathogen Nucleic Acid Isolation Kit, for HIV-1 dried blood spots (DBS) (Cat. No. A53770) different from the MagMAX Viral/Pathogen Nucleic Acid Isolation Kit (Cat. No. A42352, A48310)?

MagMAX Viral/Pathogen Nucleic Acid Isolation Kit, for HIV-1 dried blood spots (DBS) (Cat. No. A53770) and MagMAX Viral/Pathogen Nucleic Acid Isolation Kit (Cat. No. A42352, A48310) are identical, except for the extra MagMAX Dried Blood Spots Lysis Solution included in MagMAX Viral/Pathogen Nucleic Acid Isolation Kit, for HIV-1 dried blood spots (DBS) (Cat. No. A53770).

Can the MagMAX Viral/Pathogen Nucleic Acid Isolation Kit, for HIV-1 dried blood spots (DBS) also isolate host genomic DNA?

Yes,the MagMAX Viral/Pathogen Nucleic Acid Isolation Kit, for HIV-1 dried blood spots (DBS) is a total nucleic acid isolation kit.

Does the MagMAX Viral/Pathogen Nucleic Acid Isolation Kit, for HIV-1 dried blood spots (DBS) work with other pathogens or sample types?

At this time, only HIV-1 DBS (Dried Blood Spots) cards and HIV-1 plasma have been validated with the MagMAX Viral/Pathogen Nucleic Acid Isolation Kit, for HIV-1 dried blood spots (DBS).

Is the MagMAX Dried Blood Spots Lysis Solution from the MagMAX Viral/Pathogen Nucleic Acid Isolation Kit, for HIV-1 dried blood spots (DBS) sold separately?

The MagMAX Dried Blood Spots Lysis Solution is not offered as a standalone item at this time.