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查看更多产品信息 CTS™ Viral Production Cells - FAQs (A53956)
3 个常见问题解答
No, CTS Viral Production Cells are HEK 293F suspension cells that have been adapted to CTS LV-MAX Production Medium. They do not express the SV40 large T antigen.
CTS Viral Production Cells can be frozen directly in cryogenic medium consisting of 90% CTS LV-MAX Production Medium plus 10% DMSO. Here is the protocol:
- Bank cells at passage 3 when cell density reaches ˜3.0-4.5 x 10E6 viable cells/mL at >95% viability.
- Centrifuge the cells at 100 x g (low speed) for 5 mins to pellet. Discard the spent medium.
- Prepare freezing medium: e.g., 10 mL of freezing medium is 9 mL of CTS LV-MAX Production Medium + 1 mL DMSO; mix well and sterilize by 0.2 µm filtration.
- Resuspend the cell pellet in the prepared freezing medium.
- Dilute the cells to a final density of 10 x10E6 viable cells/mL and aliquot 1 mL per cryovial.
- Freeze the cells at -80 degrees C for 1 day.
- Transfer frozen vials to liquid nitrogen for long-term storage.
CTS Viral Production Cells 2.0 are specifically designed to work with CTS AAV MAX products to provide maximal adeno-associated virus (AAV) titers and optimal performance. CTS Viral Production Cells can be used but we don't recommend doing this as it will reduce system performance.