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View additional product information for TrueMark™ Respiratory I Plus Combo Kit - FAQs (A56284C)
13 product FAQs found
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The recommended specimens that can be used with the TrueMark Infectious Disease panels are listed below:
- TrueMark Respiratory I Plus Combo Kit, TrueMark Respiratory II Plus Combo Kit, and TrueMark Respiratory III Plus Combo Kit: Nasopharyngeal (NP) or nasopharyngeal aspirate swabs
- TrueMark Urinary I Plus Combo Kit, TrueMark Urinary II Plus Combo Kit, and TrueMark Urinary III Plus Combo Kit: Urine samples
- TrueMark STI Plus Combo Kit: Vaginal and genital swabs
- TrueMark Vaginal Plus Combo Kit: Vaginal swabs
- TrueMark Lesion Plus Combo Kit: Lesion/genital swab
- TrueMark Genital Plus Combo Kit: Genital swab
Here are the controls listed in the TrueMark Infectious Disease panels user guide and their considerations for use:
TrueMark Amplification Control (Cat. No. A55698) or TrueMark Amplification Control I, low conc. (Cat. No. A55699):
- Added to a single well per target in the real-time PCR plate as a positive control. The recommended concentration for the positive control is 10,000 copies/reaction.
- Monitors real-time PCR reaction setup and reagent integrity.
- Can be ordered separately.
- Includes all the targets in the TrueMark panels.
Negative (extraction) Control:
- It is a negative control on the extraction sample plate that contains water.
- The negative (extraction) control is run on the real-time PCR plate.
- Monitors cross-contamination of reagents during reaction setup.
TaqMan Universal Extraction Control Organism (B. atrophaeus) (Cat. No. A39180):
- Used only for TrueMark Urinary plates.
- Contains three vials of lyophilized Bacillus atrophaeus, for use as a process control for cell lysis and nucleic acid recovery.
- Like other gram-positive bacteria, Bacillus atrophaeus has thick cell walls that can be difficult to lyse. This characteristic makes Bacillus atrophaeus an ideal control to monitor the efficiency of cell lysis and subsequent nucleic acid recovery.
- Is supplied lyophilized with a quantity of 1 x 10E9 copies/vial and is reconstituted in 200 µL of PBS (1X), pH 7.4 to a final concentration of 5 x 10E6 copies/µL.
- The following B. atrophaeus dilution and spike-in process instructions are recommended:
Vortex for 30 seconds, until the pellet is resuspended. Make the suitable number of aliquots and stored at -20 degrees C for up to 4 months. Perform further dilution to obtain the working concentration of 5 x 10E4 copies/µL. 10 µL of the control is added to urine samples in each extraction well at the start of extraction.
It takes approximately 3 hours for running the assay starting from sample preparation to receiving the Ct value.
We recommend running the plate immediately. If the plate is not ready to run, store it at 2-8 degrees C for up to 2 hours.
We recommend using Nuclease-free water as a negative control.
There are no trial sizes or sample products available.
The recommended concentration for the positive amplification control is 10,000 copies/reaction.
- TrueMark Amplification Control I (1 x 10E5 copies/µL), Cat. No. A55699 is the recommended positive control for TrueMark Infectious Disease panels.
- TrueMark Amplification Control I (5 x 10E7 copies/µL), Cat. No. A55698 is recommended for validation purposes.
TrueMark pathogen assays on TaqMan array plates are a collection of duplex assays that detect microbes and an internal control (RNase P) in the same reaction in the RTI and STI panels. This allows for the simultaneous detection of target and internal control in the same well. This also increases confidence in results and reduces the need to run replicates, lowering cost and workflow complexity. Since the RNAse P is dried down in these plates, additional RNAse P is not required to be added to the plates.
Note: Only the RTI and STI panels include RNase P. The internal control for UTI panels (B. atrophaeus) must be purchased separately and added by the user.
Please download the user guide and lab readiness checklist to see the workflow outlining the instruments, consumables, and reagents required to run TrueMark Infectious Disease panels.
Yes, the run templates can be downloaded from the product page.
Each inventoried TrueMark Infectious Disease Combo kit includes the following components:
- TrueMark Infectious Disease panel (inventoried TaqMan Array plates): collection of duplex assays that contain primer/probe sets specific to respiratory, urinary, STI pathogens, and RNase P genomic regions
- TrueMark Infectious Disease Panels 1-Step Multiplex Master Mix (No ROX): ready-to-use PCR mix, including reverse transcriptase, polymerase, dNTPs, salt, and buffer
For more details on other consumables, please refer to the user guide or lab readiness checklist.