Applied Biosystems TrueMark STI Plus Combo 试剂盒通过实时 PCR 技术实现分子检测,利用 QuantStudio了解更多信息
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货号
数量
A56291C
5块板
货号 A56291C
价格(CNY)
-
数量:
5块板
Applied Biosystems TrueMark STI Plus Combo 试剂盒通过实时 PCR 技术实现分子检测,利用 QuantStudio 实时 PCR 系统的强大功能,为许多常见传染病领域提供端到端的工作流程。TrueMark 检测板(随附)和 QuantStudio 系统相结合,有助于快速、准确地检测和分类导致 STI 疾病的微生物。
TrueMarkSTI Plus 检测板由便利的双通路 TaqMan 测定试剂盒组成,利用实时 PCR 技术,可在同一反应中检测沙眼衣原体、淋病奈瑟氏菌、阴道毛滴虫、生殖支原体、人型支原体、微小脲原体、解脲支原体、HSV1/HSV2 和内部对照。
TrueMark STI Plus 检测板的特点包括: • 易于使用—便利的双通路反应包括测定和阳性对照(在 TaqMan 板上预先点样和干燥),这样设计旨在易于设置和提高准确度;只需添加样品和 TrueMark 传染病检测板一步法多通路预混液(无 ROX)(随附) • 内源性对照—双通路反应在每个孔中提供 RNase P 人内部对照,以提高可靠性 • 周转时间—从一系列预定义检测板中选择周转时间短的检测板 • 扩增控制—TrueMark 扩增控制 I 涵盖了 RTI、UTI、STI 和阴道检测板中的所有靶标 • 充分利用工作流程—利用相同的工作流程,从 SARS-CoV-2 检测扩展到 TrueMark 传染病研究检测板
TrueMark STI Plus Combo 试剂盒包括以下组分: • TrueMark STI Plus 检测板—一组双通路测定试剂盒,包含针对沙眼衣原体、淋病奈瑟氏菌、阴道毛滴虫、生殖支原体、人型支原体、微小脲原体、解脲支原体、HSV1/HSV2 和 RNase P 基因组区域的特异性引物/探针集 • TrueMark 传染病检测板一步法多通路预混液(无 ROX)—即用型 PCR 混合物,包括逆转录酶、聚合酶、dNTP、盐和缓冲液
产品用途 TrueMark STI Plus 检测板是一组双通路测定试剂盒,可在同一反应中检测微生物和内部对照,用于从表现出引起宫颈炎/子宫颈炎症的性传播感染体征和症状的个体的阴道拭子中,定性检测和鉴定沙眼衣原体、淋病奈瑟氏菌、阴道毛滴虫、生殖支原体、人型支原体、微小脲原体、解脲支原体、HSV1/HSV2。
规格
兼容性QuantStudio™ 5 实时 PCR 系统,96孔,0.2-mL模块;QuantStudio™ 5 Dx 实时 PCR 系统,96孔,0.2 mL 模块;QuantStudio™ 7 Pro 实时 PCR 系统,96孔,0.2 mL 模块
5块板;3 x 1 mL 将检测板储存在 15–30°C 下。 将预混液储存在 5 至 -30°C 下。
常见问题解答 (FAQ)
Does Thermo Fisher Scientific offer analytical validation (AV) consulting services?
When adopting a new testing method for your research needs, we can assist in enabling the verification of the workflow to help ensure a successful launch. Our analytical validation (AV) consulting services can speed up the launch process by up to 75% and cut costs by up to half. Our expert team will consult to help develop and optimize your research workflow, while providing control material, data analysis support, and template documentation to fully maximize your instrument and reagent investment. Please refer to https://www.thermofisher.com/av or contact your sales representative for more information.
What specimen types can be used with the TrueMark Infectious Disease panels?
The recommended specimens that can be used with the TrueMark Infectious Disease panels are listed below:
- TrueMark Respiratory I Plus Combo Kit, TrueMark Respiratory II Plus Combo Kit, and TrueMark Respiratory III Plus Combo Kit: Nasopharyngeal (NP) or nasopharyngeal aspirate swabs
- TrueMark Urinary I Plus Combo Kit, TrueMark Urinary II Plus Combo Kit, and TrueMark Urinary III Plus Combo Kit: Urine samples
- TrueMark STI Plus Combo Kit: Vaginal and genital swabs
- TrueMark Vaginal Plus Combo Kit: Vaginal swabs
- TrueMark Lesion Plus Combo Kit: Lesion/genital swab
- TrueMark Genital Plus Combo Kit: Genital swab
What are the differences between the controls listed in the TrueMark Infectious Disease panels user guide?
Here are the controls listed in the TrueMark Infectious Disease panels user guide and their considerations for use:
TrueMark Amplification Control (Cat. No. A55698) or TrueMark Amplification Control I, low conc. (Cat. No. A55699):
- Added to a single well per target in the real-time PCR plate as a positive control. The recommended concentration for the positive control is 10,000 copies/reaction.
- Monitors real-time PCR reaction setup and reagent integrity.
- Can be ordered separately.
- Includes all the targets in the TrueMark panels.
Negative (extraction) Control:
- It is a negative control on the extraction sample plate that contains water.
- The negative (extraction) control is run on the real-time PCR plate.
- Monitors cross-contamination of reagents during reaction setup.
TaqMan Universal Extraction Control Organism (B. atrophaeus) (Cat. No. A39180):
- Used only for TrueMark Urinary plates.
- Contains three vials of lyophilized Bacillus atrophaeus, for use as a process control for cell lysis and nucleic acid recovery.
- Like other gram-positive bacteria, Bacillus atrophaeus has thick cell walls that can be difficult to lyse. This characteristic makes Bacillus atrophaeus an ideal control to monitor the efficiency of cell lysis and subsequent nucleic acid recovery.
- Is supplied lyophilized with a quantity of 1 x 10E9 copies/vial and is reconstituted in 200 µL of PBS (1X), pH 7.4 to a final concentration of 5 x 10E6 copies/µL.
- The following B. atrophaeus dilution and spike-in process instructions are recommended:
Vortex for 30 seconds, until the pellet is resuspended. Make the suitable number of aliquots and stored at -20 degrees C for up to 4 months. Perform further dilution to obtain the working concentration of 5 x 10E4 copies/µL. 10 µL of the control is added to urine samples in each extraction well at the start of extraction.
What is the approximate workflow time for running the TrueMark Infectious Disease panel assay starting from sample preparation?
It takes approximately 3 hours for running the assay starting from sample preparation to receiving the Ct value.
With TrueMark Infectious Disease panels, how long can we hold the samples before starting the procedure?
We recommend running the plate immediately. If the plate is not ready to run, store it at 2-8 degrees C for up to 2 hours.