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Invitrogen™
Lyo-ready Bst DNA Polymerase
Invitrogen Lyo-ready Bst DNA Polymerase is a proprietary mutant of Bst DNA polymerase, large fragment, with a fast reaction speed, increased sensitivity, and increased tolerance to inhibitors.
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| 货号 | 数量 | 最大浓度 |
|---|---|---|
| A56655 | 1200 U | 6 U/μL |
| A56656 | 6000 U | 6 U/μL |
| A56657 | 1200 U | 40 U/μL |
货号 A56655
价格（CNY)
613.00
Each
数量:
1200 U
最大浓度:
6 U/μL
价格（CNY)
613.00
Each
Invitrogen Lyo-ready Bst DNA Polymerase is a proprietary mutant of Bst DNA polymerase, large fragment, with a fast reaction speed, increased sensitivity, and increased tolerance to inhibitors. By retaining strong strand-displacement activity, this enzyme helps enable highly efficient isothermal amplifications such as loop-mediated isothermal amplification (LAMP).
Invitrogen Lyo-ready Bst DNA Polymerase is a proprietary mutant of Bst DNA polymerase, large fragment, with a fast reaction speed, increased sensitivity, and increased tolerance to inhibitors. By retaining strong strand-displacement activity, this enzyme helps enable highly efficient isothermal amplifications such as loop-mediated isothermal amplification (LAMP).
Lyo-ready Bst DNA Polymerase possesses 5´→3´ DNA polymerase activity and lacks 5´→3´ exonuclease activity and 3´→5´ exonuclease activity. It is also a glycerol-free enzyme, enabling assay lyophilization and use in microfluidics-based technologies.
Lyo-ready Bst DNA Polymerase features
• Fast target detection in as little as 10 minutes
• Enhanced sensitivity down to 50 copies using optimized reaction buffer in (RT-)LAMP reaction
• Robust amplification, even from inhibitor-containing RNA or DNA samples
• Ability to optimize LAMP or RT-LAMP reactions to fit your needs
Applications
Lyo-ready Bst DNA Polymerase can be used for a wide variety of applications including:
• Loop-mediated isothermal amplification (LAMP)
• Whole genome amplification (WGA)
• Rolling circle amplification (RCA)
• Recombinase polymerase amplification (RPA)
• Helicase-dependent amplification (HDA)
• Exponential amplification reaction (EXPAR)
Notes
1. If performing real-time LAMP detection with SYTO 9 Green Fluorescent Nucleic Acid Stain, dilute the stock to 50 μM with nuclease-free water before every use.
2. SuperScript IV Reverse Transcriptase and RNaseOUT Recombinant Ribonuclease Inhibitor are recommended for reactions containing RNA targets.
仅供科研使用。不可用于诊断程序。
规格
最大浓度6 U/μL
反应次数200 Reactions
最佳反应温度65°C
反应时间≤10 minutes
运输条件Dry Ice
适用于（应用）LAMP, RT-LAMP
聚合酶Lyo-ready Bst DNA Polymerase
产品类型Stand-alone enzyme
数量1200 U
Unit SizeEach
内容与储存
• Lyo-ready Bst DNA Polymerase (200 μL at 6 U/μL)
• 10X Bst Reaction Buffer (1 mL)
• 200 mM MgCl2 (1 mL)
• 5 M Betaine (1 mL)
Store at –15 to –25°C.