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CultureCEPT™ Supplement (1000X), 0.5 mL - FAQs

CultureCEPT™ Supplement (1000X), 0.5 mL - FAQs

查看更多产品信息 CultureCEPT™ Supplement (1000X) - FAQs (A56800, A56799)

9 个常见问题解答

Can CultureCEPT Supplement be used with routine passaging of PSCs long-term?

Published data shows that CEPT small molecules are suitable for long-term serial passaging of hPSCs. Normal pluripotency and karyotypes are observed after 40 passages (Chen Y, et al. A versatile polypharmacology platform promotes cytoprotection and viability of human pluripotent and differentiated cells. Nat Methods. 2021 May;18(5):528-541).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can CultureCEPT Supplement effectively improve survival after cryopreservation if not included in the freezing medium?

Yes, CultureCEPT Supplement is fast acting and provides significant benefit to cells recovering from cryopreservation if introduced in the cell culture media during post-thaw recovery.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

When using CultureCEPT Supplement, does seeding density need to be adjusted?

Seeding density should be examined when using CultureCEPT Supplement to ensure optimal conditions. In many cases, the appropriate seeding density is based on the number of viable cells in each plate or well. When achieving increased viability with CultureCEPT Supplement, you will need to account for this. In many scenarios, it may be possible to plate fewer cells.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What mechanisms are involved with CEPT small molecules present in CultureCEPT Supplement?

Chroman 1 is a ROCK2 inhibitor, Emricasan is a pan-caspase inhibitor, polyamines provide essential polycations, and trans ISRIB is an integrated stress response inhibitor.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Does CultureCEPT Supplement contain animal-derived components?

No, CultureCEPT Supplement only contains small molecules.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Once thawed, can CultureCEPT Supplement be stored in a refrigerator?

Long-term storage of CultureCEPT Supplement is recommended at -20 degrees C, where the supplement is stable for at least 12 months. After thawing, the supplement can be kept at 4 degrees C for 1 week.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can I aliquot and re-freeze CultureCEPT Supplement?

Yes, CultureCEPT Supplement can be aliquoted and re-frozen. We recommend limiting to one re-freeze.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Will CultureCEPT Supplement work with different cell lines?

Yes, CultureCEPT Supplement has been evaluated on a variety of hPSC lines and different types of primary cells. Also, the small molecules of CEPT have been demonstrated to improve cell survival independent of the cell line (Chen Y, et al. A versatile polypharmacology platform promotes cytoprotection and viability of human pluripotent and differentiated cells. Nat Methods. 2021 May;18(5):528-541).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Does CultureCEPT Supplement require a specific medium?

No, CultureCEPT Supplement can work with a wide variety of media systems, such as Essential 8 Medium, Essential 8 Flex Medium, StemFlex Medium, StemScale PSC Suspension Medium, Neurobasal Plus Medium, and Neurobasal Medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.