Zeba™ Spin Desalting Plates, 40K MWCO, 4 Plates, 4 plates - FAQs

查看更多产品信息 Zeba™ Spin Desalting Columns and Plates, 40K MWCO, 75 μL–10 mL - FAQs (A57761, A57760, A40002476, A40002471, A57759, A57758, A57768, A57756, A57767, A57766, A57765, A57764, A57763, A57762)

3 个常见问题解答

Can Zeba Desalting columns, plates, and cartridges be autoclaved?

Yes, Zeba Desalting columns, plates, and cartridges can be autoclaved at 115 degrees C for 30 min.

Find additional tips, troubleshooting help, and resources within our Protein Dialysis, Desalting, and Concentration Support Center.

What reagents and conditions are Zeba Desalting columns, plates, and cartridges compatible with?

Zeba Desalting columns, plates, and cartridges are compatible with a range of reagents and conditions. They have been confirmed to be compatible with the following:
- pH range of 3-10
- Mild oxidants/reductants
- Chaotropes (stable in 8 M urea and 6 M guanidine HCl)
- Salts
- Alcohols up to 20%
- Organic solvents such as dimethyl sulfoxide (DMSO) and dimethylformamide (DMF) (we recommend using a step-gradient of increasing concentration of the organic solvent during column equilibration)

Find additional tips, troubleshooting help, and resources within our Protein Dialysis, Desalting, and Concentration Support Center.

Can I use Zeba Desalting plates with a vacuum manifold, and if so, are there any specific considerations or limitations to keep in mind?

Yes, Zeba Desalting plates can be used with a vacuum manifold. Unfortunately, we do not offer a protocol for the same. It is important to note that the pressure settings of the vacuum manifold will need to be optimized for each instrument as the pressure apparatus may differ. Additionally, it has been reported that protein recovery with Zeba Desalting plates using a vacuum manifold may be lower compared to the traditional centrifugation workflow.

Find additional tips, troubleshooting help, and resources within our Protein Dialysis, Desalting, and Concentration Support Center.