TaqMan™ Cells-to-CT™ Express Lysis Reagents

This TaqMan Cells-to-CT Express Lysis Reagents product is a convenient, large volume offering of the cell lysis reagents used in our TaqMan Cells-to-CT Express Kit. The reagents include Express Lysis Buffer and Express ezDNase. They are ideal for preparing RNA from cultured mammalian cells for gene expression analysis. The lysis procedure simultaneously prepares cell lysates for RT-qPCR and removes genomic DNA in only five minutes. The lysis step is simple to automate with robotic platforms for high-throughput processing of 96- or 384-well plates because it takes place entirely at room temperature. The procedure is also economical as there are only a few pipetting steps and, for cells grown in 96- or 384-well plates, no sample transfers.

Features of the TaqMan Cells-to-CT Express Lysis Reagents include:

• Extraordinary ease and speed—96 samples ready for RT-qPCR in as little as 5 minutes
• No tedious RNA purification—no columns, heating, or centrifugation
• Environmentally friendly—the kit is REACH Compliant, has fewer components, and requires fewer pipetting steps, saving plastics costs
• Right sized kits for automation—available 2500 reaction size includes both lysis and RT components

Optimized chemistry with no need for stop solution

Gene expression analysis from direct cell lysis is made simple with our TaqMan Cells-to-CT Express Kit. With only one step for cell lysis and no need for the addition of a Stop Solution, our Express technology enables a rapid workflow with sensitive, accurate, and reproducible results. 10–100,000 cells are lysed using the Express Lysis Solution. The optional inclusion of ezDNase to the Express Lysis Solution enables removal of genomic DNA during cell lysis. Following a 5-minute incubation at room temperature, the lysate is used directly for reverse transcription and qPCR analysis.