TMTpro™ 16plex 标签试剂组件
TMTpro™ 16plex 标签试剂组件
Thermo Scientific™

TMTpro™ 16plex 标签试剂组件

Thermo Scientific TMTpro 16plex 标记试剂套件可实现通过串联质谱 (MS/MS)，对多达 16 份样品同时的进行蛋白鉴定和定量。该 TMTpro了解更多信息
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货号数量
A5833496 次反应
A445201 x 5 mg（每个标签）
A445211 x 0.5 mg（每个标签）
A445226 x 0.5 mg（每个标签）
货号 A58334
价格（CNY)
8,221.00
Each
添加至购物车
数量:
96 次反应
价格（CNY)
8,221.00
Each
添加至购物车
Thermo Scientific TMTpro 16plex 标记试剂套件可实现通过串联质谱 (MS/MS)，对多达 16 份样品同时的进行蛋白鉴定和定量。
该 TMTpro 16plex 标记试剂套件的特点包括：
•多通路—对源自细胞、组织或生物体液的多达 16 份样品进行并行 MS 分析
• 稳健性—通路量增加使样品的缺失定量值减少，并使重复实验的可靠性提高
• 高效—胺反应性、NHS 酯活化试剂可确保高效标记所有肽，无需考虑蛋白序列或蛋白水解酶的特异性
• 兼容性—经过优化可与高分辨率 Thermo Scientific MS/MS 平台（如 Q Exactive 和 Orbitrap Fusion Tribrid 仪器系列，包括 Orbitrap Eclipse Tribrid 和 Orbitrap Exploris 480 质谱仪）配合使用，Proteome Discoverer 2.3 支持数据分析

TMTpro 标记试剂是新一代串联质谱标签，设计用于提高样品通量水平而不影响蛋白鉴定和定量的数量。TMTpro 标签结构与 TMT 标签结构类似，因为标签仍为同量异序和胺反应性，但是在长间隔区和异丁基脯氨酸质量报告基因区不同。在 MS/MS 片段化后，每个 TMTpro 标签在高分辨率 MS/MS 质谱的低质量区域生成一个唯一的报告基因质量（即 TMTpro 126-134Da），用于蛋白表达水平的相对定量。

TMTpro 标签的优势在于增加多通路相对定量、增加样品处理量、减少样品定量值的缺失。TMTpro 16plex 标记试剂适用于抑制剂剂量反应实验、时间进程实验、热转移测定或生物学重复等实验中多蛋白样品的分析。结合行业前列的高分辨率 Orbitrap 仪器和软件使用时，TMTpro 试剂可为定量蛋白表达分析提供综合全面的解决方案。

对于 TMTpro 标记肽进行高分辨率分析，Nanospray Flex 离子源推荐使用的 LC 色谱柱为 Acclaim PepMap 100 C18 LC 色谱柱（货号：164942 或 164939）。对于 EASY-Spray 离子源，推荐 LC 色谱柱为 EASY-Spray C18 LC 色谱柱（货号 ES802 或 ES803）。这些产品仅适用于应用范围有限的标签许可。

仅供科研使用。不可用于诊断程序。
规格
最终产品类型Peptide
适用于（设备）质谱仪
配方Liquid in stabilized DMSO solution
标签或染料Tandem Mass Tag™ (TMT) 标记
数量96 次反应
工作流程步骤蛋白质标记
检测方法Mass Spectrometry
形式Liquid
产品线TMT™
产品类型同量异位标记试剂套件
原始材料Cell Lysate
Unit SizeEach

常见问题解答 (FAQ)

How can I tell if the buffer in which my peptides are resuspended are compatible with TMT and TMTpro labels?

TMT and TMTpro labels are not compatible with buffers containing primary amines. After protein digestion, peptides need to be cleaned up and dried down. Prior to labeling, we do recommend resuspending the peptides in 10 µL of either 100 mM TEAB (pH 8.5) or 100 mM HEPES (pH 8.0).

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

Is the TMT stabilizing solution compatible with subsequent MS steps?

For further steps after TMT labelling, the stabilizing solution can be considered as a 33% DMSO solution. If subsequent excess TMT removal will be performed using EasyPep solid-phase extraction (SPE), the DMSO solution does not need to be diluted. On the other hand, the use of any reverse phase chromatography method (i.e., Cat. No. 84868) might need dilution of the sample to ensure that the DMSO concentration is tolerated by the resin. Alternatively, the peptide mix can be dried down using Speed Vac and then resuspended in the corresponding buffer for a particular column (Reverse Phase or Desalting).

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

With TMT 10plex, TMT 11plex, TMTpro 16plex, and TMTpro 18plex 96-well plates, how much protein can be used per TMT or TMTpro reaction?

TMT and TMTpro label reagents in 96-well plates are optimized to work with 1 to 10 µg of protein digest.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

Can the sealing foil be partially removed with TMT 10plex, TMT 11plex, TMTpro 16plex, and TMTpro 18plex 96-well plates?

Yes, the sealing foil can be partially peeled to access individual sets of TMT or TMTpro labels. Alternatively, each of the wells can be perforated to transfer the TMT/TMTpro labels to a different tube, if needed.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

How do I determine the correct orientation of the TMT 10plex, TMT 11plex, TMTpro 16plex, and TMTpro 18plex 96-well plates?

All the plates are barcoded in the long lower edge, helping to recognize correct plate orientation, plus the plate has a notch on the left corners.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

View all TMTpro™ 16plex 标签试剂组件 FAQs