TMTpro 18-plex、TMTpro-134C 和 TMTpro-135N 标记试剂
TMTpro 18-plex、TMTpro-134C 和 TMTpro-135N 标记试剂
Thermo Scientific™

TMTpro 18-plex、TMTpro-134C 和 TMTpro-135N 标记试剂

TMTpro 18plex 标记试剂实现对多达18份样品的多通路检测。添加 TMTpro 134C 和 TMTpro 135N 标记试剂可将样品多通路从16通路增加至18通路，甚至提高了通过串联质谱 (MS) 法进行蛋白鉴别和定量测定的通量。
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货号数量标签或染料
A583354 x 50 μg (per tag)Tandem Mass Tag™ (TMT) 标记
A520455 mgTMTpro 18-plex
A520465 mgTMTpro-134C and TMTpro-135N
A520476 x 0.5 mgTMTpro 18-plex
A520486 x 0.5 mgTMTpro-134C and TMTpro-135N
货号 A58335
价格（CNY)
6,666.00
Each
添加至购物车
数量:
4 x 50 μg (per tag)
标签或染料:
Tandem Mass Tag™ (TMT) 标记
价格（CNY)
6,666.00
Each
添加至购物车

TMTpro 标记试剂是新一代串联质谱标签，设计用于提高样品多通路水平，同时不影响蛋白鉴定和定量。Thermo Scientific TMTpro 18plex 标记试剂套件实现对多达18份样品的多通路检测，甚至提高了通过串联质谱 (MS) 法进行蛋白鉴别和定量分析的通量。

TMTpro 和 TMT 标签的结构在同量异位和胺反应性方面相似，但 TMTpro 标签具有更长的间隔区和异丁基脯氨酸质量报告基因。在 MS/MS 片段化之后，每个 TMTpro 18plex 标签在高分辨率 MS/MS 质谱的低质量区域生成一个独特报告基因质量（例如 TMTpro 126-135 Da），用于蛋白表达水平的相对定量测定。

TMT pro134C 和 TMTpro135N 标记试剂的组成和结构与其他 TMTpro 16plex 标签相同，但不是同量异位。添加 TMTpro 134C 和 135N 标签创建了第17和第18通道，可使用高分辨率 Orbitrap 质谱仪进行相对定量测定。这些标签单独提供（货号：A52046、A52048）或作为 TMTpro 18plex 标记试剂套件的一部分提供。

TMTpro 18plex 标记试剂套件的特点包括：
• 多通路—对源自细胞、组织或生物体液的多达18份样品进行并行 MS 分析
• 稳健—多通路能力提高，可减少样品中定量值的缺失，并提高重复试验之间的置信度
• 高效—胺反应性、NHS 酯活化试剂可确保所有肽的标记率超过 95%，无论蛋白质序列或蛋白水解酶特异性如何
• 经优化可与高分辨率 Thermo Scientific MS/MS Orbitrap 平台配合使用，并且 Proteome Discoverer 2.3 完全支持分析
• 便利的规格—以即用型、一次性使用形式提供，可确保良好稳定性和性能

为对 TMTpro 标记肽进行高分辨率分析，推荐用于 Nanospray Flex 离子源的 LC 色谱柱是 Acclaim PepMap 100 C18 LC 柱（货号：164942 或 164939）。对于 EASY-Spray 离子源，推荐 LC 色谱柱为 EASY-Spray C18 LC 色谱柱（货号 ES902 或 ES903）。结合行业领先的高分辨率 Orbitrap 仪器和软件使用时，TMTpro 试剂可为定量蛋白表达分析提供综合完整的解决方案。

仅供科研使用。不可用于诊断程序。
规格
最终产品类型Protein PEPTIDE
适用于（设备）质谱仪
配方Liquid in stabilized DMSO solution
标签或染料Tandem Mass Tag™ (TMT) 标记
数量4 x 50 μg (per tag)
足够用于72 Reactions
工作流程步骤蛋白质标记
检测方法Mass Spectrometry
形式Liquid
产品线TMT™
产品类型同量异位标记试剂套件
原始材料Cell Lysate
Unit SizeEach

常见问题解答 (FAQ)

How can I tell if the buffer in which my peptides are resuspended are compatible with TMT and TMTpro labels?

TMT and TMTpro labels are not compatible with buffers containing primary amines. After protein digestion, peptides need to be cleaned up and dried down. Prior to labeling, we do recommend resuspending the peptides in 10 µL of either 100 mM TEAB (pH 8.5) or 100 mM HEPES (pH 8.0).

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

Is the TMT stabilizing solution compatible with subsequent MS steps?

For further steps after TMT labelling, the stabilizing solution can be considered as a 33% DMSO solution. If subsequent excess TMT removal will be performed using EasyPep solid-phase extraction (SPE), the DMSO solution does not need to be diluted. On the other hand, the use of any reverse phase chromatography method (i.e., Cat. No. 84868) might need dilution of the sample to ensure that the DMSO concentration is tolerated by the resin. Alternatively, the peptide mix can be dried down using Speed Vac and then resuspended in the corresponding buffer for a particular column (Reverse Phase or Desalting).

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

With TMT 10plex, TMT 11plex, TMTpro 16plex, and TMTpro 18plex 96-well plates, how much protein can be used per TMT or TMTpro reaction?

TMT and TMTpro label reagents in 96-well plates are optimized to work with 1 to 10 µg of protein digest.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

Can the sealing foil be partially removed with TMT 10plex, TMT 11plex, TMTpro 16plex, and TMTpro 18plex 96-well plates?

Yes, the sealing foil can be partially peeled to access individual sets of TMT or TMTpro labels. Alternatively, each of the wells can be perforated to transfer the TMT/TMTpro labels to a different tube, if needed.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

How do I determine the correct orientation of the TMT 10plex, TMT 11plex, TMTpro 16plex, and TMTpro 18plex 96-well plates?

All the plates are barcoded in the long lower edge, helping to recognize correct plate orientation, plus the plate has a notch on the left corners.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

View all TMTpro 18-plex、TMTpro-134C 和 TMTpro-135N 标记试剂 FAQs